State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Plant Dis. 2019 Oct;103(10):2599-2605. doi: 10.1094/PDIS-05-18-0879-RE. Epub 2019 Jul 24.
Tobacco black shank, caused by , is one of the most notorious tobacco diseases and causes huge economic losses worldwide. Understanding the genetic variation of populations is essential to the development of disease control measures. In this research, 210 simple sequence repeat (SSR) markers for were identified, 10 of which were polymorphic among nine reference strains. We further performed population genetic analysis of 245 isolates randomly collected from tobacco fields in Chongqing for mating type, molecular variation at 14 SSR loci (four of which were identified previously), and sensitivity to the fungicide metalaxyl. The results showed that the A2 mating type was dominant and no A1 mating type isolate was discovered. SSR genotyping distinguished 245 isolates into 46 genotypes, four of which were dominant in the population. Low genotypic diversity and excess heterozygosity were common in nearly all of the populations from Chongqing. Population analysis showed that no differentiation existed among different populations. All isolates tested were highly sensitive to metalaxyl. Taken together, our results showed that the populations from tobacco fields in Chongqing belonged to a clonal lineage and were highly sensitive to metalaxyl.
烟草黑胫病菌引起的烟草黑胫病是最臭名昭著的烟草病害之一，在全球范围内造成了巨大的经济损失。了解 的种群遗传变异对于制定病害防治措施至关重要。本研究中，鉴定了 210 个 简单序列重复（SSR）标记，其中 10 个在 9 个参考菌株中具有多态性。我们进一步对从重庆烟田随机采集的 245 个 分离物进行了交配型、14 个 SSR 位点的分子变异（其中 4 个先前已鉴定）和对杀菌剂甲霜灵的敏感性的种群遗传分析。结果表明，A2 交配型占优势，未发现 A1 交配型分离物。SSR 基因型将 245 个分离物分为 46 种基因型，其中 4 种在种群中占优势。几乎所有来自重庆的种群都表现出低基因型多样性和过剩杂合性。种群分析表明，不同种群之间没有分化。所有测试的分离物对金属灵都高度敏感。总之，我们的研究结果表明，来自重庆烟田的 种群属于无性系，对金属灵高度敏感。
