Song Ruifang, Tan Yujiao, Ahmed Waqar, Zhou Guisu, Zhao Zhengxiong
College of Resources and Environment, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
College of Tobacco Science, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
BMC Microbiol. 2022 Apr 23;22(1):112. doi: 10.1186/s12866-022-02531-z.
Black shank disease caused by Phytophthora nicotianae is a serious threat to flue-cured tobacco production. Whole-plant resistance is characterized by the expression of a number of pathogenesis-related proteins, genes, and the activity of different defense-related enzymes. In this study, we investigated the activity of defense-related enzymes and expression of differentially expressed proteins through the iTRAQ technique across two flue-cured tobacco cultivars, i.e., K326 and Hongda, in response to the black shank pathogen.
Results showed that the highest disease incidence was recorded in flue-cured tobacco cultivar Hongda compared with K326, which shows that Hongda is more susceptible to P. nicotianae than K326. A total of 4274 differentially expressed proteins were detected at 0 h and after 24 h, 72 h of post-inoculation with P. nicotianae. We found that 17 proteins induced after inoculation with P. nicotianae, including pathogenesis (5), photosynthesis (3), oxidative phosphorylation (6), tricarboxylic acid cycle (1), heat shock (1), and 14-3-3 (1) and were involved in the resistance of flue-cured tobacco against black shank disease. The expression of 5 pathogenesis-related proteins and the activities of defense-related enzymes (PPO, POD, SOD, and MDA) were significantly higher in the leaves of K326 than Hongda after inoculation with P. nicotianae.
These results provide new molecular insights into flue-cured tobacco responses to P. nicotianae. It is concluded that differences in protein expressions and defense-related enzymes play an important role in developing resistance in flue-cured tobacco cultivars against black shank disease.
由烟草疫霉引起的黑胫病是烤烟生产的严重威胁。全株抗性的特征是多种病程相关蛋白、基因的表达以及不同防御相关酶的活性。在本研究中，我们通过iTRAQ技术研究了两个烤烟品种K326和宏da对黑胫病菌的防御相关酶活性和差异表达蛋白的表达情况。
结果表明，与K326相比，烤烟品种宏da的发病率最高，这表明宏da比K326更易感染烟草疫霉。在接种烟草疫霉0小时、24小时和72小时后，共检测到4274个差异表达蛋白。我们发现接种烟草疫霉后诱导产生的17种蛋白，包括病程（5种）、光合作用（3种）、氧化磷酸化（6种）、三羧酸循环（1种）、热休克（1种）和14-3-3（1种），参与了烤烟对黑胫病的抗性。接种烟草疫霉后，K326叶片中5种病程相关蛋白的表达以及防御相关酶（PPO、POD、SOD和MDA）的活性显著高于宏da。
这些结果为烤烟对烟草疫霉的反应提供了新的分子见解。得出结论，蛋白表达和防御相关酶的差异在烤烟品种对黑胫病的抗性形成中起重要作用。
