da Silva Raimundo Lopes, da Silva Jaqueline Rodrigues, Júnior Anivaldo Pereira Duarte, Marinho Patrícia Santana Barbosa, Santos Lourivaldo Silva, Teixeira Francisco Martins, Júnior José Otávio Carréra Silva, Costa Roseane Maria Ribeiro
Laboratory of Pharmaceutical Nanotechnology, College of Pharmacy, Federal University of Pará, Pará 66075-110, Brazil.
Nanobiotechnology Laboratory, Department of Genetics and Morphology, Institute of Biological Sciences, University of Brasília, Brasília 70910-900, Brazil.
Polymers (Basel). 2019 Jul 23;11(7):1226. doi: 10.3390/polym11071226.
The development of a nanoparticulate system for the carrier antigen is now an important tool in the vaccination process, since a smaller number of doses is necessary for effective immunization. Thus, in this work a nanoparticulate system using polymers of chitosan and poly (methacrylic acid) (CS-PMAA) to adsorb the Vi antigen of Typhi was developed. CS-PMAA nanoparticles with different proportions of chitosan and poly (methacrylic acid) were obtained and reached sizes from 123.9 ± 2.48 to 234.9 ± 2.66 nm, and spherical shapes were seen in transmission microscopy. At pH 7.2, the nanoparticles had a cationic surface charge that contributed to the adsorption of the Vi antigen. Qualitative analyses of the isolated Vi antigen were performed using Fourier-transform infrared spectroscopy, which indicated the presence of all the characteristic bands of the capsular polysaccharide, and nuclear magnetic resonance, which showed signals for the five hydrogens and the N-acetyl and O-acetyl groups which are characteristic of the Vi antigen structure. In the adsorption kinetics study, the Vi capsular antigen, contained in a phosphate buffer solution of pH 7.2, experienced 55% adsorption on the 1-1% (CS-PMAA) nanoparticles. The adsorption kinetics results showed the ability of the nanoparticulate system to adsorb the Vi antigen.
开发用于载体抗原的纳米颗粒系统现已成为疫苗接种过程中的一项重要工具，因为有效免疫所需的剂量较少。因此，在本研究中，开发了一种使用壳聚糖和聚（甲基丙烯酸）（CS-PMAA）聚合物吸附伤寒Vi抗原的纳米颗粒系统。获得了不同比例壳聚糖和聚（甲基丙烯酸）的CS-PMAA纳米颗粒，其尺寸为123.9±2.48至234.9±2.66nm，在透射显微镜下呈球形。在pH 7.2时，纳米颗粒具有阳离子表面电荷，有助于Vi抗原的吸附。使用傅里叶变换红外光谱对分离出的Vi抗原进行定性分析，结果表明存在荚膜多糖的所有特征谱带；使用核磁共振分析，结果显示了五个氢以及Vi抗原结构特有的N-乙酰基和O-乙酰基的信号。在吸附动力学研究中，pH 7.2的磷酸盐缓冲溶液中所含的Vi荚膜抗原在1-1%（CS-PMAA）纳米颗粒上的吸附率为55%。吸附动力学结果表明了纳米颗粒系统吸附Vi抗原的能力。
