Edde B, Vandaele S, Darmon M, Soubiran P
Laboratoire de Biochimie Cellulaire, Collège de France, Paris.
Biol Cell. 1988;62(2):157-163.
The serum of a patient suffering from myeloma was found to decorate microtubules and mitotic spindles of cultured cells. Immunoblots performed after one- and two-dimensional electrophoresis showed a reaction with a certain subset of beta-tubulin isoforms, but not with beta'- and alpha-tubulins. The tubulin subset contained both ubiquitous (beta-3) and neurospecific (beta-4,5,6) isoforms. An IgM lambda and an IgA kappa myeloma protein were found in this serum. Immunoblots performed with specific anti-isotype second antibodies showed that the tubulin subset could be evidenced using anti-mu, alpha, lambda, and kappa-specific antisera. Moreover, the tubulin subset was also evidenced using an anti-gamma second antibody. These results, which do not exclude a participation of the myeloma proteins in the anti-tubulin reactivity, indicate, however, that the antibody response was polyclonal. The same restricted specificity of all classes of anti-tubulin antibodies of this serum favours the hypothesis that the immune response of the patient was directed against an antigen sharing epitopes with tubulin rather than with tubulin itself.
发现一名骨髓瘤患者的血清可修饰培养细胞的微管和有丝分裂纺锤体。一维及二维电泳后进行的免疫印迹显示，该血清与特定的β-微管蛋白亚型发生反应，但与β'-微管蛋白和α-微管蛋白无反应。该微管蛋白亚型包含普遍存在的（β-3）和神经特异性的（β-4、5、6）亚型。在该血清中发现了一种IgM λ型和一种IgA κ型骨髓瘤蛋白。用特异性抗同种型二抗进行的免疫印迹显示，使用抗μ、α、λ和κ特异性抗血清可证实微管蛋白亚型的存在。此外，使用抗γ二抗也证实了微管蛋白亚型的存在。这些结果虽然不排除骨髓瘤蛋白参与抗微管蛋白反应，但表明抗体反应是多克隆的。该血清中所有类别的抗微管蛋白抗体具有相同的受限特异性，这支持了这样一种假说，即患者的免疫反应针对的是与微管蛋白共享表位的抗原，而非微管蛋白本身。
