Chen Hui, Chen Xinxin, Chen Jianfeng, Zhao Hong, Wang Bin, Zheng Wei, Lü Juanjuan, Du Jiang
Pediatrics Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Jul 30;39(7):816-822. doi: 10.12122/j.issn.1673-4254.2019.07.11.
To investigate the protective effect of vitamin D (VD) against hyperoxia-induced bronchopulmonary dysplasia (BPD) in newborn mice and explore the mechanism.
Thirty-six newborn mice were randomly divided into air + VD group, air + saline group, hyperoxia + VD group, and hyperoxia + saline group. In all the groups, saline or VD was administered on a daily basis intramuscular injection. After 3 weeks of treatment, the mice were weighed and cardiac blood was collected for measurement of serum VD level using ELISA, and histological examination of the lungs was performed. Radial alveolar counting (RAC) and alveolar secondary interval volume density were measured using image analysis software. The expression levels of vascular endothelial cell growth factor (VEGF) and VEGF receptor 2 (VEGFR2) in the lung tissues were detected using Western blotting.
The weight gain rate of the mice and the weight of the lungs were significantly higher in air + saline group and air + VD group than in the hyperoxia + saline group. The RAC was significantly lower in hyperoxic+saline group than that in hyperoxia+VD group ( < 0.001), and was significantly higher in hyperoxic+VD (125 times) than in hyperoxia + VD (1250 times) group ( < 0.01). The alveolar secondary protrusion count was significantly higher in hyperoxic+VD (1250 times) group than in hyperoxic+saline group ( < 0.001), and was significantly higher in hyperoxia+VD (125 times) group than in hyperoxia + VD (1250 times) group ( < 0.01). Compared with that in air + saline group, VEGFR2 expression was significantly lowered in hyperoxia+saline group ( < 0.05) and in air+VD group ( < 0.05); VEGFR2 expression was significantly higher in hyperoxia+VD (1250 times) group than in hyperoxia+saline group ( < 0.001) and hyperoxia+VD (125 times) group ( < 0.001); VEGFR2 expression was significantly higher in hyperoxia+VD (125 times) group than in hyperoxia+ saline group ( < 0.05).
In newborn mice with BPD, VD supplement can increase the weight of the lungs and promote lung maturation, and a higher concentration of VD can better protect the lungs and promote the growth of pulmonary blood vessels.
探讨维生素D（VD）对新生小鼠高氧诱导的支气管肺发育不良（BPD）的保护作用并探究其机制。
将36只新生小鼠随机分为空气+VD组、空气+生理盐水组、高氧+VD组和高氧+生理盐水组。所有组均每日通过肌肉注射给予生理盐水或VD。治疗3周后，称取小鼠体重，采集心脏血液用ELISA法测定血清VD水平，并对肺组织进行组织学检查。使用图像分析软件测量肺泡计数（RAC）和肺泡次级间隔体积密度。采用蛋白质印迹法检测肺组织中血管内皮生长因子（VEGF）和VEGF受体2（VEGFR2）的表达水平。
空气+生理盐水组和空气+VD组小鼠的体重增加率和肺重量显著高于高氧+生理盐水组。高氧+生理盐水组的RAC显著低于高氧+VD组（<0.001），高氧+VD（125倍）组的RAC显著高于高氧+VD（1250倍）组（<0.01）。高氧+VD（1250倍）组的肺泡次级突起计数显著高于高氧+生理盐水组（<0.001），高氧+VD（125倍）组的肺泡次级突起计数显著高于高氧+VD（1250倍）组（<0.01）。与空气+生理盐水组相比，高氧+生理盐水组和空气+VD组的VEGFR2表达显著降低（<0.05）；高氧+VD（1250倍）组的VEGFR2表达显著高于高氧+生理盐水组（<0.001）和高氧+VD（125倍）组（<0.001）；高氧+VD（125倍）组的VEGFR2表达显著高于高氧+生理盐水组（<0.05）。
在患有BPD的新生小鼠中，补充VD可增加肺重量并促进肺成熟，较高浓度的VD能更好地保护肺并促进肺血管生长。
