Thompson J S, Burnett D A, Cormier R A, Vaughan W P
Department of Surgery, University of Nebraska Medical Center, Creighton University School of Medicine, Omaha 68105.
Dis Colon Rectum. 1988 Jul;31(7):529-32. doi: 10.1007/BF02553726.
Plasma diamine oxidase (DAO) activity may reflect intestinal involvement in Crohn's disease. The purpose of this study was to develop a simple heparin stimulation test for assessing postheparin plasma diamine oxidase activity in Crohn's disease. Ten volunteers and five patients with Crohn's disease received 1000 units and 3000 units of heparin intravenously and plasma samples were obtained at timed intervals. Plasma DAO activity increased significantly, compared with basal values, 30 minutes after 3000 units of heparin in both volunteers (26.2 +/- 5.0 vs. 4.5 +/- 0.5 units/ml) and patients with Crohn's disease (14.6 +/- 2.0 vs. 4.0 +/- 1.1 units/ml, P less than .05) and was significantly greater in the volunteers. There was no significant increase in DAO activity after 1000 units of heparin. Plasma DAO activity increased significantly within 15 minutes after 3000 units of heparin and remained at this high level at 60 minutes. Postheparin DAO activity correlated with the integrated area under the DAO activity curve. Plasma DAO activity correlated with the Crohn's Disease Activity Index in the patients with Crohn's disease. Plasma DAO activity, 30 minutes after the intravenous administration of 3000 units of heparin, should reflect intestinal involvement in Crohn's disease.
血浆二胺氧化酶（DAO）活性可能反映克罗恩病患者的肠道受累情况。本研究的目的是开发一种简单的肝素刺激试验，用于评估克罗恩病患者肝素化后血浆二胺氧化酶的活性。10名志愿者和5名克罗恩病患者静脉注射1000单位和3000单位肝素，并在不同时间点采集血浆样本。在志愿者（26.2±5.0对4.5±0.5单位/毫升）和克罗恩病患者（14.6±2.0对4.0±1.1单位/毫升，P<0.05）中，静脉注射3000单位肝素30分钟后，血浆DAO活性与基础值相比显著增加，且志愿者的增加幅度更大。注射1000单位肝素后，DAO活性没有显著增加。静脉注射3000单位肝素后15分钟内，血浆DAO活性显著增加，并在60分钟时维持在较高水平。肝素化后DAO活性与DAO活性曲线下的积分面积相关。在克罗恩病患者中，血浆DAO活性与克罗恩病活动指数相关。静脉注射3000单位肝素30分钟后的血浆DAO活性应能反映克罗恩病患者的肠道受累情况。
