D'Agostino L, Ciacci C, Daniele B, Barone M V, Sollazzo R, Mazzacca G
Dig Dis Sci. 1987 Mar;32(3):313-7. doi: 10.1007/BF01297059.
Diamine oxidase (DAO) is an enzyme whose low plasma values are enhanced by an intravenous injection of heparin, which releases the enzyme from the enterocytes of the villous tips. In 20 normal controls and 15 untreated subjects affected with an overt malabsorption syndrome and subtotal atrophy shown by Crosby jejunal mucosa biopsy (12 suspected celiac disease and three small bowel lymphoma), plasma diamine oxidase was assayed, over 2 hr following an intravenous bolus of 15,000 IU heparin. Plasma postheparin DAO concentrations and the corresponding values of the area under curve, expressed as units/ml X min (mean +/- SD), were significantly lower in the patients (celiac sprue: 138 +/- 62; lymphoma: 83 +/- 42) compared to normals (481 +/- 104). DAO area values were well correlated (r = 0.81; P less than 0.001) with 24-hr fecal fat excretion but not with xylosuria. Our data suggest that postheparin plasma DAO assay may be useful to detect and quantitate small bowel mucosal atrophy in patients with malabsorption syndromes.
二胺氧化酶（DAO）是一种酶，静脉注射肝素可提高其较低的血浆水平，肝素能使该酶从绒毛顶端的肠细胞中释放出来。对20名正常对照者以及15名患有明显吸收不良综合征且经克罗斯比空肠黏膜活检显示有小肠萎缩（12例疑似乳糜泻和3例小肠淋巴瘤）的未经治疗的受试者，在静脉推注15000国际单位肝素后2小时以上检测血浆二胺氧化酶。与正常对照者（481±104）相比，患者（乳糜泻：138±62；淋巴瘤：83±42）的肝素后血浆DAO浓度以及相应的曲线下面积值（以单位/毫升×分钟表示，均值±标准差）显著更低。DAO面积值与24小时粪便脂肪排泄量高度相关（r = 0.81；P＜0.001），但与木糖尿无关。我们的数据表明，肝素后血浆DAO检测可能有助于检测和定量吸收不良综合征患者的小肠黏膜萎缩。
