Zhang Yu, Zhou Yunyun, Liu Xuefeng, Yu Huiliang, Li Diqiang, Zhang Yuguang
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, and the Key Laboratory of Biological Conservation of National Forestry and Grassland Administration, Beijing, 100091, China.
Hunan University of Humanities, Science and Technology, Loudi, 417000, China.
Genetica. 2019 Aug;147(3-4):327-335. doi: 10.1007/s10709-019-00073-3. Epub 2019 Jul 24.
The Sichuan snub-nosed monkey (Rhinopithecus roxellana) is an endangered species endemic to China, where the smallest population resides in Shennongjia National Park, Hubei Province. In this study, the genetic diversity of the Sichuan snub-nosed monkeys from two areas, Qianjiaping (QJP) and Dalongtan (DLT) in Shennongjia National Park was evaluated using single nucleotide polymorphisms (SNPs) data derived from restriction site-associated DNA sequencing (RAD-seq). A total of 41,260 SNP loci were found in 29 Sichuan snub-nosed monkey individuals. The genome-wide nucleotide diversity (π) of the Shennongjia population was 0.001842. The genetic differentiation (F) between the QJP and DLT subpopulations was 0.034. The heterozygosity of individuals from QJP was 0.3475 ± 0.03696 and 0.3148 ± 0.03501 for individuals from DLT. Although the DLT and QJP subpopulations did not show significant genetic differences, genetic differentiation between the two subpopulations was confirmed using Bayesian cluster analysis, neighbor-joining trees and principal component analysis. These results suggest that the Shennongjia population of Sichuan snub-nosed monkey has relatively low genetic diversity at the genomic level. The little genetic differentiation noted between the DLT and QJP subpopulations likely due to natural and anthropogenic barriers which may exacerbate loss of genetic diversity of this endangered subpopulation.
川金丝猴（Rhinopithecus roxellana）是中国特有的濒危物种，其最小种群分布在湖北省神农架国家公园。在本研究中，利用限制性位点相关DNA测序（RAD-seq）获得的单核苷酸多态性（SNP）数据，对神农架国家公园千家坪（QJP）和大龙潭（DLT）两个区域的川金丝猴的遗传多样性进行了评估。在29只川金丝猴个体中总共发现了41,260个SNP位点。神农架种群的全基因组核苷酸多样性（π）为0.001842。QJP和DLT亚种群之间的遗传分化（F）为0.034。QJP个体的杂合度为0.3475±0.03696，DLT个体的杂合度为0.3148±0.03501。虽然DLT和QJP亚种群没有显示出显著的遗传差异，但通过贝叶斯聚类分析、邻接法树和主成分分析证实了两个亚种群之间的遗传分化。这些结果表明，神农架川金丝猴种群在基因组水平上的遗传多样性相对较低。DLT和QJP亚种群之间遗传分化较小，可能是由于自然和人为障碍，这可能会加剧这个濒危亚种群遗传多样性的丧失。
