Department of Medical Imaging, Second Affiliated Hospital , Shantou University Medical College , Shantou , Guangdong 515000 , P. R. China.
CAS Key Laboratory of Brain Function and Disease, School of Life Sciences , University of Science and Technology of China , Hefei , Anhui 230000 , P. R. China.
ACS Chem Neurosci. 2019 Aug 21;10(8):3859-3867. doi: 10.1021/acschemneuro.9b00334. Epub 2019 Aug 7.
A reliable and reproducible detection of Aβ deposits would be beneficial for the early diagnosis of Alzheimer's disease (AD). In the present study, the feasibility of applying chemical exchange saturation transfer (CEST) for Aβ deposit detection using angiopep-2 as a probe was evaluated, and it was demonstrated that CEST could detect angiopep-2 and Aβ-angiopep-2 aggregates . Furthermore, APP/PS1 mice injected with angiopep-2 exhibited a significantly higher CEST effect when compared with controls. The distribution of Aβ deposits detected by CEST imaging was consistent with the histological staining results. The present study is the first to report a reliable exogenous CEST probe to noninvasively evaluate Aβ deposits in APP/PS1 mice. Furthermore, these results demonstrate the potential for clinical AD diagnosis and Aβ-targeted drug therapy assessment using CEST imaging with the angiopep-2 probe.
可靠且可重现的 Aβ 沉积检测将有助于阿尔茨海默病（AD）的早期诊断。在本研究中，评估了使用血管肽-2 作为探针应用化学交换饱和转移（CEST）检测 Aβ 沉积的可行性，结果表明 CEST 可检测血管肽-2 和 Aβ-血管肽-2 聚集体。此外，与对照组相比，注射血管肽-2 的 APP/PS1 小鼠表现出更高的 CEST 效应。CEST 成像检测到的 Aβ 沉积分布与组织学染色结果一致。本研究首次报道了一种可靠的外源性 CEST 探针，可无创评估 APP/PS1 小鼠中的 Aβ 沉积。此外，这些结果表明，使用血管肽-2 探针的 CEST 成像具有用于临床 AD 诊断和 Aβ 靶向药物治疗评估的潜力。
