State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, 066004, China.
Analyst. 2019 Aug 16;144(17):5179-5185. doi: 10.1039/c9an01053j.
The development of noble ultra-small biocompatible Pt nanoclusters (Pt NCs) for glucose detection has been drawing great attention. Herein, ultra-small biocompatible jujube polysaccharide (JP) stabilized platinum nanoclusters (Ptn-JP NCs) are prepared using natural JP as a reducing and solubilizing agent. Ptn-JP NCs were studied for the colorimetric detection of glucose. Ptn-JP NCs (n = 50, 200 and 400) had an average particle diameter of 1-2 nm. Particularly, the measurements of hydrodynamic sizes of Ptn-JP NCs indicated that they maintained good stability in solution for one week. Pt200-JP NCs showed good biocompatibility, and were not toxic against HeLa cells at a high concentration of 400 μg mL-1. Furthermore, Pt200-JP NCs catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) with H2O2 to produce blue oxidized TMB (oxTMB). This reaction followed typical Michaelis-Menten kinetics. More importantly, the glucose concentration could be sensitively detected by the color change, and this process was not interfered by other sugars. The linear range for glucose concentration was from 0.01 to 1 mM with a detection limit of 5.47 μM. The glucose concentrations of real samples of serum using Pt200-JP NCs were 9.2, 4.9 and 6.5 mM, respectively. The prepared Ptn-JP NCs have great potential in various biomedical detection methods.
超小生物相容性铂纳米簇（Pt NCs）在葡萄糖检测方面的发展引起了广泛关注。在此，我们使用天然大枣多糖（JP）作为还原剂和增溶剂，制备了超小生物相容性大枣多糖稳定的铂纳米簇（Ptn-JP NCs）。研究了 Ptn-JP NCs 用于葡萄糖的比色检测。Ptn-JP NCs（n=50、200 和 400）的平均粒径为 1-2nm。特别是，Ptn-JP NCs 的水动力粒径测量表明，它们在溶液中保持良好的稳定性，可稳定存在一周。Pt200-JP NCs 具有良好的生物相容性，在 400μgmL-1的高浓度下对 HeLa 细胞没有毒性。此外，Pt200-JP NCs 可以催化 H2O2 氧化 3,3',5,5'-四甲基联苯胺（TMB）生成蓝色氧化 TMB（oxTMB）。该反应遵循典型的米氏动力学。更重要的是，可以通过颜色变化灵敏地检测葡萄糖浓度，该过程不受其他糖的干扰。葡萄糖浓度的线性范围为 0.01-1mM，检测限为 5.47μM。使用 Pt200-JP NCs 测定血清中实际样品的葡萄糖浓度分别为 9.2、4.9 和 6.5mM。所制备的 Ptn-JP NCs 在各种生物医学检测方法中具有很大的应用潜力。
