Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, India.
Int J Pharm. 2019 Sep 10;568:118553. doi: 10.1016/j.ijpharm.2019.118553. Epub 2019 Jul 22.
Blending two polymers is an effective technique to obtain a novel material with desirable properties. Chitosan (CH) has limited applications in tissue engineering owing to its poor mechanical strength in a wet state. Polycaprolactone (PCL) has low toxicity with good mechanical strength and controlled release properties, but lack cell recognition signals. Thus, the blending of CH and PCL (CH/PCL) polymers would provide a better biomaterial required for the management of chronic osteomyelitis (OM) after surgical debridement possessing superior physicomechanical and controlled release properties. Herein, blend sponges using different ratios of CH and PCL, i.e., 100%CH/00%PCL, 75%CH/25%PCL, 50%CH/50%PCL and 25%CH/75%PCL were prepared, which are denoted as 100CH/00PCL, 75CH/25PCL, 50CH/50PCL and 25CH/75PCL, respectively. These blend sponges were characterized using FTIR, XRD, DSC, SEM, and contact angle. The results revealed that CH and PCL polymers were well dispersed in a blend at a molecular level without any chemical interactions. Blend sponges were loaded with ciprofloxacin hydrochloride (CIP) and ibuprofen. Further, in vitro efficacy of drug-loaded blend sponges was evaluated for drug release, antibacterial potential, and anti-inflammatory activity. Amongst four blend sponges, the 75CH/25PCL sponge demonstrated the controlled release of ibuprofen and an ideal release profile of CIP along with potential antibacterial as well as anti-inflammatory activity over the study period. Thus, it can be concluded that the 75CH/25PCL sponge is a promising candidate for the management of chronic OM after surgical debridement.
将两种聚合物混合是获得具有理想性能的新型材料的有效技术。壳聚糖 (CH) 在湿态下机械强度有限，因此在组织工程中的应用有限。聚己内酯 (PCL) 具有低毒性、良好的机械强度和控制释放性能，但缺乏细胞识别信号。因此，CH 和 PCL（CH/PCL）聚合物的共混将为慢性骨髓炎 (OM) 手术后清创管理提供更好的生物材料，具有优异的物理机械性能和控制释放性能。在此，使用不同比例的 CH 和 PCL 制备混合海绵，即 100%CH/00%PCL、75%CH/25%PCL、50%CH/50%PCL 和 25%CH/75%PCL，分别表示为 100CH/00PCL、75CH/25PCL、50CH/50PCL 和 25CH/75PCL。这些共混海绵采用 FTIR、XRD、DSC、SEM 和接触角进行表征。结果表明，CH 和 PCL 聚合物在分子水平上很好地分散在共混物中，没有任何化学相互作用。共混海绵负载盐酸环丙沙星 (CIP) 和布洛芬。此外，还评估了载药共混海绵的体外药效，包括药物释放、抗菌潜力和抗炎活性。在四种共混海绵中，75CH/25PCL 海绵表现出布洛芬的控制释放以及 CIP 的理想释放曲线，同时具有潜在的抗菌和抗炎活性。因此，可以得出结论，75CH/25PCL 海绵是慢性 OM 手术后清创管理的有前途的候选材料。
