University of Belgrade, Innovation Centre, Faculty of Technology and Metallurgy, Karnegijeva 4, 11120 Belgrade, Serbia.
University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11120 Belgrade, Serbia.
Eur J Pharm Sci. 2018 Nov 1;124:26-36. doi: 10.1016/j.ejps.2018.08.023. Epub 2018 Aug 18.
Antibiotic containing polycaprolactone (PCL) fibers were produced by using three electrospinning methods: blend, emulsion and co-axial electrospinning (labeled as S1, S2 and S3, respectively). The profiles of drug release from three different systems were studied and antimicrobial properties of produced materials were evaluated. Morphology of the produced fibers was characterized and revealed that cefazolin-loaded PCL fibers had smaller diameter compared to neat PCL fibers, while the chemical interaction between the antibiotic and PCL showed that cefazolin neither had reacted with PCL phase, nor had degraded during the electrospinning process. The crystallinity and thermal characterization of fabricated fibers showed that the addition of cefazolin decreased the crystallinity of PCL. The results of the drug release behavior of the blend and co-axial electrospun fibers was on a higher level (68% and ~43%, respectively) compared to the emulsion electrospun fibers (5%), after a period of 30 days. The obtained data had the best fitting with the first order model and the Higuchi model, while the Korsmeyer-Peppas model showed a Pseudo-Fickian diffusion of the drug. Antibacterial evaluations showed that cefazolin-loaded PCL fibers had better effects on Staphylococcus aureus compared to Escherichia coli during the treatment period and that the effect of the emulsion fibers was notably weaker than the other two studied systems. The aim of the study was to test different systems for control drug release of different dynamics, which will be applied for prevent bacterial accumulation when indwelling urinary catheters, applied for different periods of time.
共混、乳液和同轴静电纺丝（分别标记为 S1、S2 和 S3）制备了含抗生素的聚己内酯（PCL）纤维。研究了三种不同体系的药物释放曲线，并评价了所制备材料的抗菌性能。对所制备纤维的形貌进行了表征，结果表明，载头孢唑林的 PCL 纤维的直径小于纯 PCL 纤维，而抗生素与 PCL 之间的化学相互作用表明，头孢唑林既没有与 PCL 相反应，也没有在静电纺丝过程中降解。所制备纤维的结晶度和热特性表明，头孢唑林的加入降低了 PCL 的结晶度。与乳液静电纺丝纤维（约 5%）相比，共混和同轴静电纺丝纤维的药物释放行为（分别约为 68%和 43%）在 30 天的时间内处于较高水平。获得的数据与一级模型和 Higuchi 模型拟合度最好，而 Korsmeyer-Peppas 模型则显示药物呈假 Fickian 扩散。抗菌评价表明，在治疗期间，载头孢唑林的 PCL 纤维对金黄色葡萄球菌的效果优于大肠杆菌，而乳液纤维的效果明显弱于另外两种研究体系。本研究的目的是测试不同体系以控制不同动力学的药物释放，这将应用于预防留置导尿管时细菌的积累，应用于不同的时间段。
