IL-6、IL-10 和 TGF-β1 基因多态性与布鲁氏菌病的关联：系统评价与荟萃分析。

Association of IL-6, IL-10 and TGF-β1 gene polymorphisms with brucellosis: A systematic review with meta-analysis.

机构信息

Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.

Experimental Teaching Center, School of Public Health of Southwest Medical University, Luzhou, 646000, China.

出版信息

Microb Pathog. 2019 Oct;135:103640. doi: 10.1016/j.micpath.2019.103640. Epub 2019 Jul 22.

DOI:10.1016/j.micpath.2019.103640

PMID:31344477

Abstract

BACKGROUND

Brucellosis is one of the major public health problems worldwide. Several current studies have provided data that polymorphisms in the interleukin-6 (IL-6), interleukin-10 (IL-10) and transforming growth factor beta1(TGF-β1) gene were associated with the susceptibility to human brucellosis, but the results remain inconsistent.

OBJECTIVES

The aim of present study was to investigate the relationship between IL-6 (-174 G/C), IL-10 (-1082 A/G, -819C/T) and TGF-β1 (codon 10, codon 25) gene polymorphisms and brucellosis.

METHODS

We performed a comprehensive search of the PubMed, EMBASE, Web of Science, OVID-EBMR, and the Cochrane Library up to Oct. 30, 2018. The search was designed using the following key words: "brucellosis" or" "brucella melitensis", "IL-10" or "interleukin10" or "interleukin-10", "IL-6" or "interleukin6" or "interleukin-6", "TGF-β1" or "TGF-beta1" or "transforming growth factor β1", "polymorphism" and "single nucleotide polymorphism (SNP)". Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to measure the strength of association between TGF-β1, IL-10 and IL-6 polymorphisms and brucellosis risk. All the statistical analyses were conducted by Review manager 5.3 software.

RESULTS

A total of 8 studies involving 1308 cases and 902 controls met the inclusion criteria for IL-6, IL-10, TGF-β1 polymorphisms and brucellosis risk. There was a slightly trend of increasing risk of brucellosis in individuals with the G allele compared with individuals with the C allele (OR = 1.07, 95% CI: 0.85-1.33, P = 0.57) in IL-6 polymorphism. However, statistical analysis showed that these differences are not significant. Our results suggested TGF-β1 (codon 25 G/C) GG genotype may be considered as a risk factor for brucellosis (OR = 1.67, 95% CI: 1.12-2.50, P = 0.01). Herein, we failed to find any significant association between IL-10 (-1082 A/G, -819C/T), TGF-β1 (codon 10C/T) gene polymorphism and susceptibility to brucellosis in all gene models.

CONCLUSION

IL-6 (-174 G/C), IL-10 (-1082 A/G, -819C/T), and TGF-β1 (codon 10C/T) polymorphisms is not a risk factor for brucellosis infection. TGF-β1 codon 25 GG genotype may be considered as a risk factor for brucellosis.

摘要

背景

布氏杆菌病是全球主要的公共卫生问题之一。目前的几项研究提供的数据表明，白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和转化生长因子β1（TGF-β1）基因中的多态性与人类布氏杆菌病的易感性有关，但结果仍不一致。

目的

本研究旨在探讨 IL-6（-174 G/C）、IL-10（-1082 A/G、-819C/T）和 TGF-β1（密码子 10、密码子 25）基因多态性与布氏杆菌病的关系。

方法

我们对 PubMed、EMBASE、Web of Science、OVID-EBMR 和 Cochrane Library 进行了全面检索，截至 2018 年 10 月 30 日。搜索使用了以下关键词：“布氏杆菌病”或“布鲁氏菌 melitensis”、“IL-10”或“白细胞介素 10”或“白细胞介素-10”、“IL-6”或“白细胞介素 6”或“白细胞介素-6”、“TGF-β1”或“TGF-β1”或“转化生长因子β1”、“多态性”和“单核苷酸多态性（SNP）”。使用 95%置信区间（CI）的粗比值比（OR）来衡量 TGF-β1、IL-10 和 IL-6 多态性与布氏杆菌病风险之间的关联强度。所有的统计分析都是用 Review Manager 5.3 软件进行的。

结果

共有 8 项研究共纳入 1308 例病例和 902 例对照，符合纳入标准，用于研究 IL-6、IL-10、TGF-β1 多态性与布氏杆菌病风险的关系。在 IL-6 多态性中，与 C 等位基因相比，G 等位基因的个体患布氏杆菌病的风险略有增加（OR=1.07，95%CI：0.85-1.33，P=0.57）。然而，统计分析表明这些差异并不显著。我们的结果表明，TGF-β1（密码子 25 G/C）GG 基因型可能是布氏杆菌病的一个危险因素（OR=1.67，95%CI：1.12-2.50，P=0.01）。在此，我们未能在所有基因模型中发现 IL-10（-1082 A/G、-819C/T）、TGF-β1（密码子 10C/T）基因多态性与布氏杆菌病易感性之间存在任何显著关联。