Bravo María José, Colmenero Juan Dios, Queipo-Ortuño María Isabel, Alonso Antonio, Caballero Abelardo
Immunology Service, Carlos Haya Regional University Hospital, Avda. Carlos Haya no 82, 29010 Malaga, Spain.
Cytokine. 2008 Oct;44(1):18-21. doi: 10.1016/j.cyto.2008.07.008. Epub 2008 Sep 18.
Polymorphisms in the cytokine genes have allowed for the understanding of the genetic determinants in several diseases. We investigated the polymorphism of the transforming growth factor (TGF)-beta1 and IL-6 genes in relation to susceptibility to human brucellosis. We typed 82 Spanish brucellosis patients and 102 healthy controls for TGF-beta1 polymorphisms in codons 10 and 25, and IL-6 promoter polymorphism at position -174 by PCR-SSP methods. The T/T G/G genotype of the TGF-beta1 gene was significantly increased in patients compared to controls (49% vs. 32%) P=0.02; OR=1.99 (1.05-3.80) and the T/C G/G genotype was significantly less common in the patients compared to the controls (32% vs. 49%) P=0.01; OR=0.48 (0.25-0.92). The CC genotype of codon 10 was significantly increased in the patients who had focal forms of the disease as compared with those who did not develop focal forms (19% vs. 4%), P=0.03; OR=0.19 (0.02-1.10). No differences were found in the IL-6 variants between the patients and the controls. These results suggest that polymorphism of the TGF-beta1 gene may be involved in susceptibility to brucellosis and to developing focal forms of the disease in a group of patients from southern Spain.
细胞因子基因多态性有助于了解多种疾病的遗传决定因素。我们研究了转化生长因子（TGF）-β1和白细胞介素-6（IL-6）基因多态性与人类布鲁氏菌病易感性的关系。我们采用聚合酶链反应-序列特异性引物（PCR-SSP）方法，对82例西班牙布鲁氏菌病患者和102例健康对照者进行了TGF-β1基因第10和25密码子的多态性以及IL-6基因启动子-174位点多态性的分型。与对照组相比，患者中TGF-β1基因的T/T G/G基因型显著增加（49%对32%），P = 0.02；比值比（OR）= 1.99（1.05 - 3.80），且T/C G/G基因型在患者中显著少于对照组（32%对49%），P = 0.01；OR = 0.48（0.25 - 0.92）。与未出现局灶性病变的患者相比，患有局灶性病变的患者中第10密码子的CC基因型显著增加（19%对4%），P = 0.03；OR = 0.19（0.02 - 1.10）。患者与对照组之间的IL-6基因变体未发现差异。这些结果表明，TGF-β1基因多态性可能与西班牙南部一组患者的布鲁氏菌病易感性以及疾病局灶性病变的发生有关。
