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评价牙椅用水管路中微生物和自由生活原生动物的污染情况。

Evaluation of Microbiological and Free-Living Protozoa Contamination in Dental Unit Waterlines.

机构信息

Department of Health Sciences, University of Genova, Via Pastore 1, 16132 Genova, Italy.

Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, 00133 Rome, Italy.

出版信息

Int J Environ Res Public Health. 2019 Jul 24;16(15):2648. doi: 10.3390/ijerph16152648.

DOI:10.3390/ijerph16152648
PMID:31344972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6696308/
Abstract

Studies conducted over the last 40 years have demonstrated that the water output from dental unit waterlines (DUWLs) is often contaminated with high densities of microorganisms. It has been monitored the microbiological quality of the water in 30 public dental facilities in northern Italy in order to assess the health risk for patients and dental staff. In each facility, samples of water both from taps and from DUWLs were analyzed in order to evaluate heterotrophic plate counts (HPCs) at 22 °C and 36 °C, and to detect coliform bacteria, , and amoebae. In 100% of the samples taken from the DUWLs, the concentration of HPCs was above the threshold as determined by the Ministère de la Santé et des Solidarités (2007). The concentration of P. aeruginosa was greater than the indicated threshold in 16.67% of the hand-pieces analyzed. A total of 78.33% of samples were contaminated by , while in the samples taken from the DUWLs alone, this percentage rose to 86.67%. Amoebae were detected in 60% of the samples taken from hand-pieces; all belonging to the species . This study documented the presence of various microorganisms, including spp., at considerably higher concentrations in water samples from DUWLs than in samples of tap water in the same facilities, confirming the role of the internal DUWLs in increasing microbial contamination, especially in the absence of proper management of waterborne health risks.

摘要

过去 40 年来的研究表明,牙科手机供水系统(DUWL)的出水中经常存在高密度的微生物。为了评估患者和牙科医护人员的健康风险,对意大利北部 30 家公共牙科机构的水质进行了监测。在每个机构中,都对来自水龙头和 DUWL 的水样进行了分析,以评估 22°C 和 36°C 时的异养平板计数(HPC),并检测大肠菌群、假单胞菌属和阿米巴虫。在从 DUWL 中采集的水样中,100%的 HPC 浓度超过了 Ministère de la Santé et des Solidarités(2007 年)规定的阈值。在分析的手机中,16.67%的铜绿假单胞菌浓度超过了指示阈值。共有 78.33%的样本被检测出含有,而在仅从 DUWL 中采集的样本中,这一比例上升到 86.67%。在从手机中采集的 60%的样本中检测到了阿米巴虫;所有这些都属于种。本研究记录了各种微生物的存在,包括铜绿假单胞菌属等,在 DUWL 水样中的浓度明显高于同一设施中自来水样中的浓度,证实了内部 DUWL 在增加微生物污染方面的作用,尤其是在没有对水传播健康风险进行适当管理的情况下。

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Vermamoeba vermiformis: a Free-Living Amoeba of Interest.
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