Wang Yong, Wu Jing-Zhen, Li Yu, Qi Xu
Department of Pharmacy, Jiangsu Jianhu People's Hospital, Yancheng 224700, China.
School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Evid Based Complement Alternat Med. 2019 Jun 27;2019:2089586. doi: 10.1155/2019/2089586. eCollection 2019.
Perimenopause symptoms have an extremely high incidence in aging women. Development of new strategies to improve perimenopause symptoms is important topic in clinical context. Increasing studies have shown that the polysaccharides of (PFC) have many pharmacological activities including antiaging effects. Here, we evaluated the effects of PFC on the ovarian function in natural aging-associated perimenopause symptoms in mice.
Natural aging mice (16-month old) were orally administrated with PFC at 1.11 g/kg daily for 24 days with none-treated young mice (3-month old) as control. Blood samples were collected for measurements of serum levels of estradiol, progesterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH). Ovaries were isolated for histopathological and molecular exanimations.
We found that the aging mice had decreased number of growing follicles and corpus luteum in ovary, but treatment with PFC restored their amounts. Measurement of hormones showed that there were low serum levels of estradiol and progesterone but high levels of LH and FSH in aging mice; however PFC restored estradiol and progesterone levels but reduced LH and FSH levels. Immunohistochemical analysis with ovarian tissues also revealed that the expression of inhibin and insulin-like growth factor 1 was reduced in the ovary of aging mice but was restored by PFC. These data indicated that PFC regulated ovarian function-associated hormone levels in aging mice. Furthermore, there was reduced expression of antiapoptotic protein Bcl-2 and increased expression of proapoptotic molecules Bax and cleaved-caspase-3 in the ovary of aging mice. However, treatment with PFC upregulated Bcl-2 and downregulated Bax and cleaved-caspase-3, suggesting that PFC inhibited apoptosis of granulosa cells in the ovary of aging mice.
PFC improved the ovarian function in mice, which had high potential to be developed as a safe and effective therapeutic remedy for aging-associated perimenopause symptoms.
围绝经期症状在老年女性中发病率极高。开发改善围绝经期症状的新策略是临床领域的重要课题。越来越多的研究表明，[具体物质名称]多糖（PFC）具有多种药理活性，包括抗衰老作用。在此，我们评估了PFC对自然衰老相关围绝经期症状小鼠卵巢功能的影响。
将自然衰老小鼠（16月龄）以1.11 g/kg的剂量每日口服PFC，持续24天，以未处理的年轻小鼠（3月龄）作为对照。采集血样以测定血清雌二醇、孕酮、黄体生成素（LH）和卵泡刺激素（FSH）水平。分离卵巢进行组织病理学和分子检查。
我们发现衰老小鼠卵巢中生长卵泡和黄体数量减少，但PFC治疗可使其数量恢复。激素测定显示，衰老小鼠血清雌二醇和孕酮水平较低，但LH和FSH水平较高；然而，PFC可恢复雌二醇和孕酮水平，但降低LH和FSH水平。对卵巢组织的免疫组化分析还显示，衰老小鼠卵巢中抑制素和胰岛素样生长因子1的表达降低，但PFC可使其恢复。这些数据表明，PFC调节衰老小鼠卵巢功能相关激素水平。此外，衰老小鼠卵巢中抗凋亡蛋白Bcl-2的表达降低，促凋亡分子Bax和裂解的半胱天冬酶-3的表达增加。然而，PFC治疗上调了Bcl-2并下调了Bax和裂解的半胱天冬酶-3，表明PFC抑制了衰老小鼠卵巢颗粒细胞的凋亡。
PFC改善了小鼠的卵巢功能，极有可能被开发成为一种安全有效的治疗衰老相关围绝经期症状的药物。
