González-Rizo Aileen, Castañet Camilo E, Companioni Ariamys, Menéndez Zulema, Hernández Hilda, Magdalena-Rodríguez M, Gato Rene
Departamento Control de Vectores, Centro de Investigación Diagnóstico y Referencia, Instituto de Medicina Tropical "Pedro Kourí", La Habana, Cuba.
Facultad de Biología, Universidad de La Habana, La Habana, Cuba.
J Arthropod Borne Dis. 2019 Mar 30;13(1):39-49. eCollection 2019 Mar.
The efficacy of biolarvicides may be influenced by species of mosquito, larval age and density, temperature, water quality, bacterial formulation, and others. The aim of this study was to evaluate the influence of temperature and chlorine on larvicidal activity of Cuban isolates against .
The influence of temperature (25, 30, 35 °C) and chlorine (2.25mg/L) on the larvicidal activity of eleven Cuban isolates (collected between 2007 and 2009) were tested under laboratory conditions following WHO protocols. Bioassay data were analyzed by Probit program. The effect of chlorine and temperature (25, 30, 35 and 40 °C) on the Cry and Cyt proteins of these isolates was determined by SDS-PAGE polyacrylamide gel electrophoresis.
The pathogenicity of the isolates U81, X48 was affected at 35 °C. However, A21, A51, L910, and R89 isolates increase their entomopathogen activity at 35 °C. No differences were observed in toxicity of M29, R84, R85 and R87 isolates at different temperatures. The Cry 4, Cry 10 and Cry 11 proteins were reduced in A21, X48, R85 isolates at 35 and 40 °C. The Cyt proteins were reduced at 35 and 40 °C in A21, X48, R85, and A51 isolates. In L910 and R84 isolates, the Cyt toxin was degraded only at 40 °C. In chlorinated water, the lethal concentrations 50 and 90 in A21, A51, M29, R84, U81, and X48 isolates were increase.
A21, A51, L910, R85, and X48 isolates have a strong larvicidal activity for the treatment of breeding's sites exposed to high temperature and chlorine.
生物杀幼虫剂的功效可能受蚊子种类、幼虫年龄和密度、温度、水质、细菌制剂等因素影响。本研究旨在评估温度和氯对古巴分离株杀灭 幼虫活性的影响。
按照世界卫生组织规程，在实验室条件下测试温度（25、30、35℃）和氯（2.25mg/L）对11株古巴分离株（于2007年至2009年收集）杀灭幼虫活性的影响。生物测定数据通过概率程序进行分析。通过SDS - PAGE聚丙烯酰胺凝胶电泳确定氯和温度（25、30、35和40℃）对这些分离株的Cry和Cyt蛋白的影响。
分离株U81、X48在35℃时致病性受到影响。然而，分离株A21、A51、L910和R89在35℃时昆虫病原活性增强。在不同温度下，分离株M29、R84、R85和R87的毒性未观察到差异。在35℃和40℃时，分离株A21、X48、R85中的Cry 4、Cry 10和Cry 11蛋白减少。在35℃和40℃时，分离株A21、X48、R85和A51中的Cyt蛋白减少。在L910和R84分离株中，Cyt毒素仅在40℃时降解。在氯化水中，分离株A21、A51、M29、R84、U81和X48的致死浓度50和90增加。
分离株A21、A51、L910、R85和X48对处理暴露于高温和氯的孳生地具有很强的杀幼虫活性。
