在伊朗东部扎博勒使用PCR方法鉴定绵羊和媒介蜱中的物种

Identification of Species in Sheep and Vector Ticks Using PCR Method in Zabol, Eastern Iran.

作者信息

Zarei Fateme, Ganjali Maryam, Nabavi Reza

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.

出版信息

J Arthropod Borne Dis. 2019 Mar 30;13(1):76-82. eCollection 2019 Mar.

BACKGROUND

is a protozoal parasite that belongs to the phylum Apicomplexa. Theileriosis is an important tick-borne disease caused by various species of . Among these species, () is highly pathogenic, while other species such as make Subclinical and mild infections in small ruminant. Therefore, the precise identification of the species and the vector ticks are very essential for epidemiological studies and the design of control programs.

METHODS

This research was conducted with the aim of molecular study to identify species and vectors in Zabol, eastern Iran in 2015. The presence of in 80 blood samples and vector ticks was evaluated using PCR method.

RESULTS

Of 80 blood samples, PCR analysis showed that 50 samples (62.5%) were infected with The evaluation of the first phase PCR with Nested PCR showed that infections with and were 67.45% and 32.55% cases respectively. Overall, 110 ticks (78 males and 32 females) were collected and generally two genera and six species including (9.1%), (29.1%), (10.9%) (23.63%), (10.9%), (16.37%) were detected. After evaluating ticks infection by PCR method, three species of , and , were infected.

CONCLUSION

has a high prevalence among the sheep of zabol and , and may be the main vectors of species in this area.

背景

是一种属于顶复门的原生动物寄生虫。泰勒虫病是由多种引起的重要蜱传疾病。在这些种类中，（）具有高致病性，而其他种类如在小反刍动物中引起亚临床和轻度感染。因此，准确鉴定该物种和传播媒介蜱对于流行病学研究和控制方案的设计非常重要。

方法

本研究旨在通过分子研究鉴定2015年伊朗东部扎博勒的物种和传播媒介。使用PCR方法评估80份血液样本和传播媒介蜱中是否存在。

结果

在80份血液样本中，PCR分析显示50份样本（62.5%）感染了。巢式PCR对第一阶段PCR的评估显示，感染和的病例分别为67.45%和32.55%。总体而言，共收集到110只蜱（78只雄性和32只雌性），共检测到两个属六个种，包括（9.1%）、（29.1%）、（10.9%）、（23.63%）、（10.9%）、（16.37%）。通过PCR方法评估蜱的感染情况后，发现、和三种蜱被感染。