Aydin Mehmet Fatih, Aktas Munir, Dumanli Nazir
Research Laboratory, Higher Health School, University of Karamanoğlu Mehmetbey, 70100, Karaman, Turkey,
Parasitol Res. 2015 Jan;114(1):65-9. doi: 10.1007/s00436-014-4160-x. Epub 2014 Sep 28.
A molecular survey was undertaken in the Black Sea region of Turkey to determine the presence of Theileria and Babesia species of medical and veterinary importance. The ticks were removed from sheep and goats, pooled according to species and locations, and analyzed by PCR-based reverse line blot (RLB) and sequencing. A total of 2241 ixodid ticks belonging to 5 genus and 12 species were collected and divided into 310 pools. Infection rates were calculated as the maximum likelihood estimation (MLE) with 95% confidence intervals (CI). Of the 310 pools tested, 46 (14.83%) were found to be infected with Theileria or Babesia species, and the overall MLE of the infection rate was calculated as 2.27% (CI 1.67-2.99). The MLE of the infection rates were calculated as 0.691% (CI 0.171-1.78) in Haemaphysalis parva, 1.47% (CI 0.081-6.37) in Rhipicephalus sanguineus, 1.84% (CI 0.101-7.87) in Ixodes ricinus, 2.86% (CI 1.68-4.48) in Rhipicephalus turanicus, 5.57% (CI 0.941-16.3) in Hyalomma marginatum, and 6.2% (CI 4.02-9.02) in Rhipicephalus bursa. Pathogens identified in ticks included Theileria ovis, Babesia ovis, Babesia bigemina, and Babesia microti. Most tick pools were infected with a single pathogen. However, five pools displayed mixed infections with T. ovis and B. ovis. This study provides the first molecular evidence for the presence of B. microti in ticks in Turkey.
在土耳其黑海地区开展了一项分子调查，以确定具有医学和兽医重要性的泰勒虫属和巴贝斯虫属物种的存在情况。从绵羊和山羊身上采集蜱虫，根据物种和地点进行合并，然后通过基于聚合酶链反应（PCR）的反向线印迹（RLB）和测序进行分析。共收集到2241只属于5个属12个物种的硬蜱，并分为310组。感染率通过最大似然估计（MLE）及95%置信区间（CI）来计算。在检测的310组中，有46组（14.83%）被发现感染了泰勒虫属或巴贝斯虫属物种，感染率的总体MLE计算为2.27%（CI 1.67 - 2.99）。微小牛蜱的感染率MLE计算为0.691%（CI 0.171 - 1.78），血红扇头蜱为1.47%（CI 0.081 - 6.37），蓖麻硬蜱为1.84%（CI 0.101 - 7.87），图兰扇头蜱为2.86%（CI 1.68 - 4.48），边缘璃眼蜱为5.57%（CI 0.941 - 16.3），柏氏扇头蜱为6.2%（CI 4.02 - 9.02）。在蜱虫中鉴定出的病原体包括绵羊泰勒虫、绵羊巴贝斯虫、双芽巴贝斯虫和微小巴贝斯虫。大多数蜱虫组感染了单一病原体。然而，有五组显示绵羊泰勒虫和绵羊巴贝斯虫的混合感染。本研究提供了土耳其蜱虫中存在微小巴贝斯虫的首个分子证据。
