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γ干扰素诱导的转录激活由蛋白激酶C介导。

Interferon-gamma-induced transcriptional activation is mediated by protein kinase C.

作者信息

Fan X D, Goldberg M, Bloom B R

机构信息

Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.

出版信息

Proc Natl Acad Sci U S A. 1988 Jul;85(14):5122-5. doi: 10.1073/pnas.85.14.5122.

DOI:10.1073/pnas.85.14.5122
PMID:3134657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC281700/
Abstract

Interferon-gamma (IFN-gamma) regulates a variety of biological functions and is the principal lymphokine known to activate macrophages. In studies of the molecular mechanisms by which these cells are regulated by IFN-gamma, the transcriptional activation of an IFN-gamma-inducible gene, gamma.1, in human macrophage-like cell lines was examined. Transcription of this gene is rapidly induced by 0.1-1 unit of IFN-gamma. In addition, gamma.1 transcription is efficiently induced by phorbol 12-myristate 13-acetate, which is known to activate protein kinase C (PKC). Both stimulators of gamma.1 transcription induce the translocation of PKC from the cytosol of a membrane fraction. Two selective inhibitors of PKC, H7 and sphingosine, suppressed not only the induction of gamma.1 mRNA but transcription of HLA-DR by IFN-gamma as well. These findings establish that PKC plays a significant role in the signal transduction pathway leading to transcriptional activation of some IFN-gamma-regulated genes of cells of the mononuclear phagocyte lineage.

摘要

γ干扰素（IFN-γ）调节多种生物学功能，是已知可激活巨噬细胞的主要淋巴因子。在研究这些细胞受IFN-γ调节的分子机制时，检测了人巨噬细胞样细胞系中IFN-γ诱导基因γ.1的转录激活情况。该基因的转录可被0.1 - 1单位的IFN-γ迅速诱导。此外，佛波酯12-肉豆蔻酸酯13-乙酸酯（已知可激活蛋白激酶C（PKC））能有效诱导γ.1转录。γ.1转录的两种刺激物均可诱导PKC从膜部分的胞质溶胶中易位。PKC的两种选择性抑制剂H7和鞘氨醇不仅抑制γ.1 mRNA的诱导，还抑制IFN-γ诱导的HLA-DR转录。这些发现表明，PKC在导致单核吞噬细胞系细胞某些IFN-γ调节基因转录激活的信号转导途径中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/63f7a703ce31/pnas00293-0187-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/dbac3a59bce1/pnas00293-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/030fb4341ae3/pnas00293-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/7e9b48fb03e0/pnas00293-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/63f7a703ce31/pnas00293-0187-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/dbac3a59bce1/pnas00293-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/030fb4341ae3/pnas00293-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/7e9b48fb03e0/pnas00293-0187-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1b2/281700/63f7a703ce31/pnas00293-0187-c.jpg

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Lymphokine enhances oxygen-independent activity against intracellular pathogens.淋巴因子增强针对细胞内病原体的非氧依赖性活性。
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Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.淋巴细胞有丝分裂原和血小板衍生生长因子对c-myc基因的细胞特异性调控。
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Expression of HLA-DR by a human monocyte cell line is under transcriptional control.人单核细胞系HLA - DR的表达受转录控制。
Fish monocytes as a model for mycobacterial host-pathogen interactions.
鱼类单核细胞作为分枝杆菌宿主-病原体相互作用的模型。
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Heat-killed Streptococcus suis capsular type 2 strains stimulate tumor necrosis factor alpha and interleukin-6 production by murine macrophages.热灭活的2型猪链球菌菌株可刺激小鼠巨噬细胞产生肿瘤坏死因子α和白细胞介素-6。
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Group B streptococci persist inside macrophages.B族链球菌在巨噬细胞内持续存在。
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Phagocytosis of the malarial pigment, hemozoin, impairs expression of major histocompatibility complex class II antigen, CD54, and CD11c in human monocytes.疟色素（疟原虫血色素）的吞噬作用会损害人类单核细胞中主要组织相容性复合体II类抗原、CD54和CD11c的表达。
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Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells.γ干扰素对人细胞中HLA抗原及其mRNA合成的优先作用。
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