Boyce Ceejay L, Willis Stephaney, Archampong Timothy N A, Lartey Margaret, Sagoe Kwamena W, Obo-Akwa Adjoa, Kenu Ernest, Kwara Awewura, Blackard Jason T
Division of Digestive Disease, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Department of Medicine and Therapeutics, School of Medicine and Dentistry, College of Health Sciences, University of Ghana, Accra, Ghana.
Virus Genes. 2019 Oct;55(5):707-712. doi: 10.1007/s11262-019-01690-y. Epub 2019 Jul 25.
Hepatitis B virus (HBV) exhibits a high degree of heterogeneity with at least 10 genotypes (A-J) identified to date. Intergenotypic recombination is relatively common. Previously, we investigated HBV drug resistance in HIV/HBV co-infected individuals in Ghana. After identifying multiple circulating genotypes and a novel D/E recombinant, we sought to determine if additional individuals were also infected with recombinant HBV. Partial genome sequences from three individuals were initially identified as genotype A4. Full-length HBV genomes were obtained using rolling circle amplification followed by PCR and shown to cluster with known A/E recombinant viruses. Similar recombination breakpoints were observed in these three individuals suggesting local spread of this novel recombinant HBV in Ghana.
乙型肝炎病毒（HBV）表现出高度的异质性，迄今为止已鉴定出至少10种基因型（A - J）。基因型间重组相对常见。此前，我们在加纳对HIV/HBV合并感染个体的HBV耐药性进行了研究。在鉴定出多种流行基因型和一种新型D/E重组体后，我们试图确定是否还有其他个体也感染了重组HBV。最初，来自三名个体的部分基因组序列被鉴定为A4基因型。使用滚环扩增随后进行PCR获得了全长HBV基因组，并显示它们与已知的A/E重组病毒聚类。在这三名个体中观察到了相似的重组断点，表明这种新型重组HBV在加纳存在局部传播。
