Hess Sonja
PEL, California Institute of Technology, Pasadena, CA, USA.
Dynamic Omics, Antibody Discovery and Protein Engineering, AstraZeneca, Gaithersburg, MD, USA.
Methods Mol Biol. 2019;2030:263-275. doi: 10.1007/978-1-4939-9639-1_20.
The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields.To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents such as Marfey's reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.
常见和特殊氨基酸立体化学的测定在食品化学、考古学、医学以及包括海洋生物学和外星化学等不同领域的生命科学中都很重要，并且极大地促进了我们目前在这些领域的知识积累。为了测定氨基酸的立体化学，在过去几十年里已经开发并完善了许多色谱方法。在此，我们描述一种基于间接色谱的先进液相色谱-质谱联用方法。氨基酸与手性衍生试剂（如马费试剂（FDAA）、GITC、S-NIFE和OPA-IBLC）反应形成非对映异构体，并使用与质谱兼容的缓冲液在反相柱上进行分离。
