Department of Proteomics and Metabolomics, Research Center for Advanced Medicine-MEDFUTURE, "Iuliu Hațieganu" University of Medicine and Pharmacy Cluj-Napoca, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania.
Department of Pharmaceutical Analysis, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania.
Int J Mol Sci. 2022 Jul 4;23(13):7428. doi: 10.3390/ijms23137428.
Chiral metabolomics is starting to become a well-defined research field, powered by the recent advances in separation techniques. This review aimed to cover the most relevant advances in indirect enantioseparations of endogenous metabolites that were published over the last 10 years, including improvements and development of new chiral derivatizing agents, along with advances in separation methodologies. Moreover, special emphasis is put on exciting advances in separation techniques combined with mass spectrometry, such as chiral discrimination by ion-mobility mass spectrometry together with untargeted strategies for profiling of chiral metabolites in complex matrices. These advances signify a leap in chiral metabolomics technologies that will surely offer a solid base to better understand the specific roles of enantiomeric metabolites in systems biology.
手性代谢组学正逐渐成为一个明确的研究领域，其发展得益于最近分离技术的进步。本综述旨在涵盖过去 10 年中发表的关于内源性代谢物间接对映体分离的最相关进展，包括手性衍生试剂的改进和新发展，以及分离方法的进展。此外，特别强调了与质谱相结合的分离技术的令人兴奋的进展，例如与非靶向策略相结合的通过离子淌度质谱进行手性区分，以在复杂基质中对手性代谢物进行分析。这些进展标志着手性代谢组学技术的飞跃，必将为更好地理解对映体代谢物在系统生物学中的特定作用提供坚实的基础。
