Department of Food Engineering, Faculty of Engineering, Niğde Ömer Halisdemir University, Niğde, Turkey.
Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey.
J Basic Microbiol. 2019 Oct;59(10):1049-1062. doi: 10.1002/jobm.201900102. Epub 2019 Jul 25.
The aim of this study was to identify and characterize the SE-P3, P16, P37, and P47 phages infecting Salmonella Enteritidis. Transmission electron microscopy analysis showed that the SE phages belonged to the Myoviridae or Siphoviridae family and had plaque sizes between 0.622 ± 0.027 and 1.630 ± 0.036 mm in diameter. sefC, pefA, spvC, sopE, and gipA virulent gene regions were absent in their genome and their calculated genome sizes were between 35.9 and 37.8 kbp. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the protein profiles of each phage were different. The SE phages had a short latent period (10-20 min), large burst size (76-356 PFU/cell), and a short burst time (25-35 min). The multiplicity of infection values and mutant frequency of the phages were 0.01-0.0001 and 10 , respectively. They were very infective against their host bacteria when applied at 20°C, 30°C, or 37°C and adsorbed to their host cells by 96.20-97.65% in the first 5 min of incubation, and also Ca ions did not have a significant effect on their adsorption. The SE phages were resistant to wide pH ranges and high temperatures. These results indicate that the SE phages are good candidates as therapeutic and biocontrol agents against foodborne pathogenic S. Enteritidis.
本研究旨在鉴定和描述感染肠炎沙门氏菌的 SE-P3、P16、P37 和 P47 噬菌体。透射电子显微镜分析表明，SE 噬菌体属于肌尾噬菌体科或长尾噬菌体科，噬菌斑大小在 0.622±0.027 和 1.630±0.036 毫米之间。sefC、pefA、spvC、sopE 和 gipA 毒力基因区域在其基因组中缺失，其基因组大小计算在 35.9 到 37.8 kbp 之间。十二烷基硫酸钠聚丙烯酰胺凝胶电泳显示，每种噬菌体的蛋白质图谱不同。SE 噬菌体潜伏期短（10-20 分钟），爆发量很大（76-356 PFU/细胞），爆发时间短（25-35 分钟）。噬菌体的感染复数和突变频率分别为 0.01-0.0001 和 10。它们在 20°C、30°C 或 37°C 时对宿主细菌非常具有感染力，在孵育的前 5 分钟内以 96.20-97.65%的速度吸附到宿主细胞上，并且 Ca 离子对其吸附没有显著影响。SE 噬菌体对宽 pH 范围和高温具有抗性。这些结果表明，SE 噬菌体是治疗和生物防治食源性病原体肠炎沙门氏菌的候选者。
