Stem Cell Research Center, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, Korea.
Mol Reprod Dev. 2019 Sep;86(9):1116-1125. doi: 10.1002/mrd.23227. Epub 2019 Jul 25.
Allicin, a chemical component of garlic, has strong antioxidant activity and is thought to exert antiaging effects in vitro. We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. The rates of oocyte survival and polar body extrusion in samples treated with 1 µM allicin (1 AL) were significantly higher than in untreated samples (0 AL). In addition, 1 AL prevented defects in spindle formation and chromosome alignment, as well as decreases in the expression of maturation markers, during in vitro aging. In this study, we considered allicin to be a regulator of autophagy rather than an antioxidant or antiapoptotic agent. At the embryo level, although the cleavage rate after parthenogenetic activation was similar in all groups, the blastocyst formation rate was higher in the 1 AL group than in the 0 AL group. Our findings demonstrate that allicin effectively prevents the deterioration of porcine oocytes during aging in vitro, and could therefore be used to improve the quality of aged oocytes used in in vitro experiments.
大蒜中的一种化学物质——蒜素，具有很强的抗氧化活性，被认为具有体外抗衰老作用。我们研究了蒜素处理是否能保护猪卵母细胞和胚胎免受排卵后由凋亡和自噬介导的老化。用 1µM 蒜素（1 AL）处理的样品中卵母细胞的存活率和第一极体排出率明显高于未处理的样品（0 AL）。此外，1 AL 可防止体外老化过程中纺锤体形成和染色体排列的缺陷，以及成熟标记物表达的减少。在这项研究中，我们认为蒜素是自噬的调节剂，而不是抗氧化剂或抗凋亡剂。在胚胎水平上，虽然各组的孤雌激活后的卵裂率相似，但 1 AL 组的囊胚形成率高于 0 AL 组。我们的研究结果表明，蒜素可有效防止猪卵母细胞在体外老化过程中的恶化，因此可用于提高体外实验中老化卵母细胞的质量。
