Yoon Jae-Wook, Lee Seung-Eun, Park Yun-Gwi, Kim Won-Jae, Park Hyo-Jin, Park Chan-Oh, Kim So-Hee, Oh Seung-Hwan, Lee Do-Geon, Pyeon Da-Bin, Kim Eun-Young, Park Se-Pill
Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 63243, Korea.
Stem Cell Research Center, Jeju National University, Jeju 63243, Korea.
Anim Biosci. 2021 Apr;34(4):546-557. doi: 10.5713/ajas.20.0046. Epub 2020 May 12.
If fertilization does not occur within a specific period, the quality of unfertilized oocytes in the oviduct (in vivo aging) or in culture (in vitro aging) will deteriorate over time. Icariin (ICA), found in all species of Epimedium herbs, has strong antioxidant activity, and is thought to exert anti-aging effects in vitro. We asked whether ICA protects oocytes against age-related changes in vitro.
We analyzed the reactive oxygen species (ROS) levels and expression of antioxidant, maternal, and estrogen receptor genes, and along with spindle morphology, and the developmental competence and quality of embryos in the presence and absence of ICA.
Treatment with 5 μM ICA (ICA-5) led to a significant reduction in ROS activity, but increased mRNA expression of glutathione and antioxidant genes (superoxide dismutase 1 [SOD1], SOD2, peroxiredoxin 5, and nuclear factor erythroid 2-like 2), during aging in vitro. In addition, ICA-5 prevented defects in spindle formation and chromosomal alignment, and increased mRNA expression of cytoplasmic maturation factor genes (bone morphogenetic protein 15, cyclin B1, MOS proto-oncogene, serine/threonine kinase, and growth differentiation factor-9). It also prevented apoptosis, increased mRNA expression of antiapoptotic genes (BCL2-like 1 and baculoviral IAP repeat-containing 5), and reduced mRNA expression of pro-apoptotic genes (BCL2 antagonist/killer 1 and activation of caspase-3). Although the maturation and cleavage rates were similar in all groups, the total cell number per blastocyst and the percentage of apoptotic cells at the blastocyst stage were higher and lower, respectively, in the control and ICA-5 groups than in the aging group.
ICA protects oocytes against damage during aging in vitro; therefore, it can be used to improve assisted reproductive technologies.
如果在特定时期内未发生受精，输卵管内未受精的卵母细胞（体内老化）或培养中的卵母细胞（体外老化）的质量会随时间而恶化。淫羊藿属植物中均含有的淫羊藿苷（ICA）具有强大的抗氧化活性，被认为在体外具有抗衰老作用。我们研究了ICA是否能在体外保护卵母细胞免受与年龄相关的变化影响。
我们分析了在有或没有ICA的情况下，活性氧（ROS）水平、抗氧化、母体和雌激素受体基因的表达，以及纺锤体形态、胚胎的发育能力和质量。
用5μM ICA（ICA-5）处理可显著降低体外老化过程中的ROS活性，但增加了谷胱甘肽和抗氧化基因（超氧化物歧化酶1 [SOD1]、SOD2、过氧化物酶体增殖物激活受体5和核因子红细胞2样2）的mRNA表达。此外，ICA-5可防止纺锤体形成和染色体排列缺陷，并增加细胞质成熟因子基因（骨形态发生蛋白15、细胞周期蛋白B1、MOS原癌基因、丝氨酸/苏氨酸激酶和生长分化因子-9）的mRNA表达。它还可防止细胞凋亡，增加抗凋亡基因（BCL2样1和含杆状病毒IAP重复序列5）的mRNA表达，并降低促凋亡基因（BCL2拮抗剂/杀手1和半胱天冬酶-3激活）的mRNA表达。尽管所有组的成熟率和裂解率相似，但对照组和ICA-5组中每个囊胚的总细胞数和囊胚期凋亡细胞的百分比分别高于和低于老化组。
ICA可保护卵母细胞在体外老化过程中免受损伤；因此，它可用于改善辅助生殖技术。
