Division of Virology, University of the Free State, Bloemfontein, South Africa.
Division of Virology, National Health Laboratory Service, Bloemfontein, South Africa.
J Med Virol. 2020 Feb;92(2):227-233. doi: 10.1002/jmv.25556.
Human papillomaviruses (HPVs) have been associated with a subset of head and neck squamous cell carcinomas (HNSCCs). The aim of this study was to determine the prevalence of HPV DNA in archived formalin-fixed paraffin-embedded tissue from patients with histologically confirmed HNSCCs in a South African cohort. A nested PCR was used for the detection of HPV DNA targeting the L1 gene. Positive samples were confirmed using an in-house hemi-nested PCR targeting the E6 gene and genotyped by sequence determination of amplicons. HPV DNA was detected in 57/780 (7.3%) samples, with the highest prevalence being in the sinonasal tract (16.0%) and oropharynx (10.8%). HPV16 was the most frequently detected type, being found in 26/57 (45.6%) positive samples. The prevalence of HPV DNA in HNSCCs found in this study was lower than that found in developed countries.
人乳头瘤病毒（HPV）与一部分头颈部鳞状细胞癌（HNSCC）有关。本研究旨在确定南非队列中经组织学证实的 HNSCC 患者存档福尔马林固定石蜡包埋组织中 HPV DNA 的流行率。使用针对 L1 基因的巢式 PCR 检测 HPV DNA。使用针对 E6 基因的内部半巢式 PCR 对阳性样本进行确认，并通过扩增子序列确定进行基因分型。在 780 个样本中检测到 57 个（7.3%）HPV DNA，其中鼻窦（16.0%）和口咽（10.8%）的阳性率最高。HPV16 是最常检测到的类型，在 26/57（45.6%）阳性样本中发现。本研究中发现的 HNSCC 中 HPV DNA 的流行率低于发达国家。
