一种用于氨基酸脱羧酶的连续分光光度测定法。

A continual spectrophotometric assay for amino acid decarboxylases.

作者信息

Scriven F, Wlasichuk K B, Palcic M M

机构信息

Department of Food Science, University of Alberta, Edmonton, Canada.

出版信息

Anal Biochem. 1988 May 1;170(2):367-71. doi: 10.1016/0003-2697(88)90644-6.

DOI:10.1016/0003-2697(88)90644-6

PMID:3134831

Abstract

A spectrophotometric method for assaying the activity of three amino acid decarboxylases is reported. This method makes use of the coupled reaction of the decarboxylase with phosphoenolpyruvate carboxylase and malate dehydrogenase. The assay is simple and rapid and allows continuous monitoring of the reaction progress. The kinetic parameters obtained using this method for diaminopimelate decarboxylase, lysine decarboxylase, and arginine decarboxylase are comparable to values obtained by radiochemical methods.

摘要

报道了一种用于测定三种氨基酸脱羧酶活性的分光光度法。该方法利用了脱羧酶与磷酸烯醇丙酮酸羧化酶和苹果酸脱氢酶的偶联反应。该测定方法简单快速，可连续监测反应进程。使用该方法获得的二氨基庚二酸脱羧酶、赖氨酸脱羧酶和精氨酸脱羧酶的动力学参数与通过放射化学方法获得的值相当。