Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Korean Collection for Type Cultures (KCTC), KoreaResearch Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3230-3236. doi: 10.1099/ijsem.0.003614.
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, HSMS-29, was isolated from a marine sand sample collected from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain HSMS-29 fell within the clade comprising the type strains of species. Strain HSMS-29 exhibited 16S rRNA gene sequence similarities of 97.2-98.4 % to the type strains of , , , , , , and and 96.3-96.9 % to the type strains of the other species. The genomic ANI values of strain HSMS-29 with the type strains of , , , , , and were 72.66-74.99 %. The DNA-DNA relatedness value between strain HSMS-29 and the type strain of was 17 %. Strain HSMS-29 contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. The major polar lipids of strain HSMS-29 were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain HSMS-29 was 65.0 mol% (HPLC) or 64.4 % (genome analysis). Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain HSMS-29 is separated from recognized species. On the basis of the data presented here, strain HSMS-29 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-29 (=KACC 19870=NBRC 113549).
一株革兰氏阴性、需氧、不运动、呈卵形或杆状的细菌菌株 HSMS-29 从韩国黄海的海洋沙样中分离得到。16S rRNA 基因序列的邻接法系统发育树显示，菌株 HSMS-29 属于 种的型菌株聚类。菌株 HSMS-29 与 种的型菌株的 16S rRNA 基因序列相似性为 97.2-98.4%，与其他 种的型菌株的 16S rRNA 基因序列相似性为 96.3-96.9%。菌株 HSMS-29 与 种的型菌株的基因组平均核苷酸同一性（ANI）值为 72.66-74.99%。菌株 HSMS-29 与 种的型菌株的 DNA-DNA 同源性值为 17%。菌株 HSMS-29 含有 Q-10 作为主要的泛醌和 C 7 作为主要的脂肪酸。菌株 HSMS-29 的主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的胺脂和一种未鉴定的脂类。菌株 HSMS-29 的 DNA G+C 含量为 65.0 mol%（HPLC）或 64.4%（基因组分析）。表型特征、系统发育和遗传特性的明显差异表明，菌株 HSMS-29 与公认的 种分离。基于目前提供的数据，菌株 HSMS-29 被认为代表了一个新的 属种，建议将其命名为 sp. nov.。其模式菌株为 HSMS-29（=KACC 19870=NBRC 113549）。
