Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3109-3115. doi: 10.1099/ijsem.0.003596.
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated MYP11, was isolated from seawater around Jeju island, Republic of Korea and identified by polyphasic taxonomic study. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain MYP11 joined the cluster comprising the type strains of , and , showing 16S rRNA gene sequence similarities of 96.3-96.8 %. Strain MYP11 exhibited 16S rRNA gene sequence similarity values of 94.2-94.9 % to the type strains of other species. In the upgma dendrogram based on the average nucleotide identity values of genomic sequences, strain MYP11 formed an evolutionary lineage independent of those of species and other taxa. Strain MYP11 contained Q-10 as the predominant ubiquinone and C 7 and cyclo C 8 as the major fatty acids. The major polar lipids of strain MYP11 were phosphatidylcholine, phosphatidylglycerol, two unidentified lipids and one unidentified aminolipid. The DNA G+C content of strain MYP11 was 63.1 or 61.5 mol%. The differences in the fatty acid and polar lipid profiles and DNA G+C contents made it reasonable to distinguish strain MYP11 from the type strains of , and and those of other species. On the basis of the polyphasic data presented here, strain MYP11 is considered to constitute a new genus and species within the class , for which the name gen. nov., sp. nov. is proposed. The type strain is MYP11 (=KCTC 62899=NBRC 113544).
一株革兰氏阴性、需氧、不运动、球形、卵形或杆状的细菌菌株，被命名为 MYP11，从韩国济州岛周围的海水中分离得到，并通过多相分类学研究进行了鉴定。16S rRNA 基因序列的邻接法系统发育树显示，菌株 MYP11 与 、 和 的模式菌株聚类在一起，16S rRNA 基因序列相似性为 96.3-96.8%。菌株 MYP11 与其他 种的模式菌株的 16S rRNA 基因序列相似性为 94.2-94.9%。基于基因组序列平均核苷酸同一性值的 upgma 系统发育树，菌株 MYP11 与 种和其他分类群的进化枝独立形成。菌株 MYP11 含有 Q-10 作为主要的泛醌，C7 和环 C8 作为主要的脂肪酸。菌株 MYP11 的主要极性脂为磷脂酰胆碱、磷脂酰甘油、两种未鉴定的脂类和一种未鉴定的胺脂。菌株 MYP11 的 DNA G+C 含量为 63.1 或 61.5mol%。脂肪酸和极性脂谱以及 DNA G+C 含量的差异使得菌株 MYP11 可以与 、 和其他 种的模式菌株以及其他 种区分开来。基于这里呈现的多相数据，菌株 MYP11 被认为构成了一个新属和新种，属于 纲，建议采用新属名 gen. nov.，新种名 sp. nov.。模式菌株为 MYP11(=KCTC 62899=NBRC 113544)。
