Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, PR China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Toxicol In Vitro. 2019 Dec;61:104607. doi: 10.1016/j.tiv.2019.104607. Epub 2019 Jul 23.
Seleno-β-lactoglobulin (Se-β-Lg) was synthesized using seleninic acid, an organoselenium compound, and β-lactoglobulin (β-Lg), an important component of milk. Previously, we have studied the effects of Se-β-Lg on hepatocellular carcinoma and gastric cancer cells. In this study, we investigated the antitumor effects of Se-β-Lg and its potential mechanisms of action against human breast cancer cells (MCF-7 and MDA-MB-231). The results showed that the half-maximal inhibitory concentrations (IC50) of Se-β-Lg were 40.84 μg/mL for MCF-7 cells and 46.04 μg/mL for MDA-MB-231 cells at 24 h, while the compound showed no cytotoxicity to normal breast cells. The involvement of reactive oxygen species (ROS) in the activation of the apoptotic signaling pathway by Se-β-Lg was demonstrated by the incubation of cells with 80 μg/mL Se-β-Lg and determination of the rates of apoptosis and intracellular ROS levels after the addition of 10 mM N-acetyl-l-cysteine, a ROS inhibitor. Our findings revealed highly potent anticancer activities of Se-β-Lg against breast cancer cells and suggested that the compound may be used as a chemopreventive agent for breast cancer. Furthermore, we thoroughly elucidated the antitumor mechanism of Se-β-Lg.
硒-β-乳球蛋白（Se-β-Lg）是使用亚硒酸（一种有机硒化合物）和β-乳球蛋白（β-Lg）合成的，β-Lg 是牛奶的重要成分。此前，我们已经研究了 Se-β-Lg 对肝癌和胃癌细胞的影响。在这项研究中，我们研究了 Se-β-Lg 的抗肿瘤作用及其对人乳腺癌细胞（MCF-7 和 MDA-MB-231）的潜在作用机制。结果表明，Se-β-Lg 在 24 小时时对 MCF-7 细胞的半最大抑制浓度（IC50）为 40.84μg/ml，对 MDA-MB-231 细胞的 IC50 为 46.04μg/ml，而该化合物对正常乳腺细胞没有细胞毒性。通过用 80μg/ml Se-β-Lg 孵育细胞并在添加 10mM N-乙酰-l-半胱氨酸（ROS 抑制剂）后测定细胞凋亡率和细胞内 ROS 水平，证明了 ROS 参与了 Se-β-Lg 激活凋亡信号通路。我们的研究结果揭示了 Se-β-Lg 对乳腺癌细胞具有很强的抗癌活性，并表明该化合物可用作乳腺癌的化学预防剂。此外，我们彻底阐明了 Se-β-Lg 的抗肿瘤机制。
