Medical and Surgical Laboratory Animal Research and Practice Center, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
Interdisciplinary Laboratory of Clinical Pathology, Interlab-UMU, University of Murcia, 30100, Murcia, Spain.
Res Vet Sci. 2019 Aug;125:290-297. doi: 10.1016/j.rvsc.2019.07.002. Epub 2019 Jul 9.
Endotoxemia treatment options are still of interest due to high mortality and choline treatment is one of them because of its role in the cholinergic anti-inflammatory pathway. This study investigated serum choline and butyrylcholinesterase (BChE) responses, and their correlations with inflammatory, oxidative stress and tissue damage biomarkers, including paraoxanase-1 (PON1), and clinical signs in calves with endotoxemia and the effect of choline treatment in these responses. Healthy calves (n = 20) were divided equally into 4 groups: Control (0.9% NaCl, iv), Choline (C; 1 mg/kg/iv,once), Lipopolysaccharide (LPS; 2 μg/kg/iv,once) and LPS + C. Clinical and laboratory examinations were performed before and 0.5-48 h (hrs) after treatments. Following LPS administration, serum choline level increased at 0.5-24 h (P < .01), whereas serum BChE and PON1 level decreased at 48 h (P < .01) compared to their baselines. In LPS + C group, the increase in serum choline level was significantly higher (P < .01) than that of C and LPS groups. LPS did not decrease serum BChE levels significantly in calves treated with choline. Serum choline and BChE results correlated negatively with white blood cell count and positively (P < .001) with PON1 levels, oxidative stress index, inflammation and hepato-muscular injury markers. In conclusion serum choline and BChE may have a role in the pathophysiology of endotoxemia in calves. High serum choline concentration is associated with an improvement in response to LPS administration in calves treated with choline, probably by preventing the imbalances between oxidative stress and anti-oxidant capacity, preventing the serum BChE and PON1 decreases, and inhibition/attenuation of acute phase reaction and hepato-muscular injury in calves with endotoxemia.
内毒素血症的治疗方法仍然受到关注，因为其死亡率很高，胆碱治疗是其中之一，因为它在胆碱能抗炎途径中起作用。本研究调查了血清胆碱和丁酰胆碱酯酶（BChE）的反应，及其与炎症、氧化应激和组织损伤生物标志物（包括对氧磷酶 1（PON1））的相关性，以及内毒素血症牛的临床症状，并研究了胆碱治疗对内毒素血症牛这些反应的影响。将 20 头健康牛平均分为 4 组：对照组（0.9%NaCl，iv）、胆碱组（C；1mg/kg/iv，一次）、脂多糖组（LPS；2μg/kg/iv，一次）和 LPS+C 组。在治疗前和治疗后 0.5-48 小时（hrs）进行临床和实验室检查。LPS 给药后，血清胆碱水平在 0.5-24 小时（P<.01）升高，而血清 BChE 和 PON1 水平在 48 小时（P<.01）时与基线相比下降。在 LPS+C 组中，与 C 组和 LPS 组相比，血清胆碱水平的升高更为显著（P<.01）。胆碱治疗组 LPS 未显著降低血清 BChE 水平。血清胆碱和 BChE 结果与白细胞计数呈负相关，与 PON1 水平呈正相关（P<.001），与氧化应激指数、炎症和肝肌损伤标志物呈正相关。结论：血清胆碱和 BChE 可能在牛内毒素血症的病理生理学中起作用。高血清胆碱浓度与牛对内毒素处理的反应改善有关，可能是通过防止氧化应激和抗氧化能力之间的失衡，防止血清 BChE 和 PON1 下降，以及抑制/减轻牛内毒素血症时的急性期反应和肝肌损伤。
