Department of Internal Medicine, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey.
Medical and Surgical Experimental Animal Practice and Research Center, Eskisehir Osmangazi University, 26480, Eskisehir, Turkey.
Vet Immunol Immunopathol. 2021 Mar;233:110197. doi: 10.1016/j.vetimm.2021.110197. Epub 2021 Jan 29.
Nasal secretory fluid proteomes (NSPs) can provide valuable information about the physiopathology and prognosis of respiratory tract diseases. This study aimed to determine changes in NSP by using proteomics in calves treated with lipopolysaccharide (LPS) or LPS + choline. Healthy calves (n = 10) were treated with LPS (2 μg/kg/iv). Five minutes after LPS injection, the calves received a second iv injection with saline (n = 5, LPS + saline group) or saline containing 1 mg/kg choline (n = 5, LPS + choline group). Nasal secretions were collected before (baseline), at 1 h and 24 h after the treatments and analysed using label-free liquid chromatography-tandem mass spectrometry (LCMS/MS). Differentially expressed proteins (>1.2-fold-change) were identified at the different time points in each group. A total of 52 proteins were up- and 46 were downregulated at 1 h and 24 h in the LPS + saline group. The upregulated proteins that showed the highest changes after LPS administration were small ubiquitin-related modifier-3 (SUMO3) and glutathione peroxidase-1 (GPX1), whereas the most downregulated protein was E3 ubiquitin-protein ligase (TRIM17). Treatment with choline reduced the number of upregulated (32 proteins) and downregulated proteins (33 proteins) in the NSPs induced by LPS. It can be concluded that the proteome composition of nasal fluid in calves changes after LPS, reflecting different pathways, such as the activation of the immunological response, oxidative stress, ubiquitin pathway, and SUMOylation. Choline treatment alters the NSP response to LPS.
鼻分泌物蛋白质组（NSP）可以提供有关呼吸道疾病生理病理学和预后的有价值的信息。本研究旨在通过使用蛋白质组学来确定用脂多糖（LPS）或 LPS+胆碱处理的小牛的 NSP 变化。将健康的小牛（n=10）用 LPS（2μg/kg/iv）处理。在 LPS 注射后 5 分钟，小牛接受第二次静脉内注射生理盐水（n=5，LPS+生理盐水组）或含有 1mg/kg 胆碱的生理盐水（n=5，LPS+胆碱组）。在处理前（基线）、处理后 1 小时和 24 小时收集鼻分泌物，并使用无标记液相色谱-串联质谱（LCMS/MS）进行分析。在每个组的不同时间点鉴定出差异表达的蛋白质（>1.2 倍变化）。在 LPS+生理盐水组中，1 小时和 24 小时时，共有 52 种蛋白质上调和 46 种蛋白质下调。在 LPS 给药后显示最高变化的上调蛋白是小泛素相关修饰物-3（SUMO3）和谷胱甘肽过氧化物酶-1（GPX1），而下调蛋白是 E3 泛素蛋白连接酶（TRIM17）。胆碱处理减少了 LPS 诱导的 NSP 中上调（32 种蛋白）和下调（33 种蛋白）的蛋白数量。可以得出结论，小牛鼻液的蛋白质组组成在 LPS 后发生变化，反映了不同的途径，如免疫反应的激活、氧化应激、泛素途径和 SUMO 化。胆碱处理改变了 NSP 对 LPS 的反应。
