Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China; Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100083, China.
Eve Energy Ltd, Guangdong 516006, China.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109748. doi: 10.1016/j.msec.2019.109748. Epub 2019 May 17.
It is well known that the differentiation of stem cells is affected by the cell culture medium, the scaffold surface and electrochemical signals. However, stimulation of patterned biomaterials seeded with stem cell cultures has not been explored. Herein the effect of electrical stimulation on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) cultured on solid and nanoporous micropyramid patterned Si surfaces was evaluated. It was found that both stimulation and scaffold patterning significantly enhanced osteo-differentiation. The stimulated nanoporous micropyramid scaffolds were more promising compared to the stimulated solid micropyramid surfaces, as they significantly promoted the osteogenic differentiation of rBMSCs via BMP/Smad signaling pathway. Particularly, as compared to the unstimulated patterned biomaterials, the stimulated patterned scaffolds allowed for a significant increase in core binding factor alpha l, alkaline phosphatase, the alpha l chain of type I Col, osteocalcin, and osteonectin, all of which are characteristic for osteo-differentiation. The proposed combination of electrical stimulation with scaffold patterning may provide novel promising strategies for bone tissue engineering and regenerative medicine.
众所周知，干细胞的分化受到细胞培养介质、支架表面和电化学信号的影响。然而，带有干细胞培养物的图案生物材料的刺激作用尚未得到探索。本文评估了在固体和纳米多孔微金字塔图案化 Si 表面上培养的大鼠骨髓间充质干细胞（rBMSCs）的电刺激对成骨分化的影响。结果发现，刺激和支架图案化都显著增强了成骨分化。与刺激的固体微金字塔表面相比，刺激的纳米多孔微金字塔支架更有前途，因为它们通过 BMP/Smad 信号通路显著促进了 rBMSCs 的成骨分化。特别是与未受刺激的图案生物材料相比，受刺激的图案化支架允许核心结合因子α l、碱性磷酸酶、I 型 Col 的α l 链、骨钙素和骨粘连蛋白的显著增加，所有这些都是成骨分化的特征。电刺激与支架图案化的结合可能为骨组织工程和再生医学提供新的有前途的策略。
