粘红酵母对L-葡萄糖的转运

Transport of L-glucose by Rhodotorula glutinis.

作者信息

Pinkerton M D, Ritchie C K, Griffin C C

机构信息

Hughes Laboratories, Department of Chemistry, Oxford.

出版信息

Biochimie. 1988 Feb;70(2):183-5. doi: 10.1016/0300-9084(88)90059-4.

DOI:10.1016/0300-9084(88)90059-4

PMID:3134941

Abstract

The kinetics of L-glucose transport by Rhodotorula glutinis were studied over a 720-fold range of sugar concentrations. Analysis of the saturation isotherm revealed the presence of a one-carrier system for L-glucose in the plasma membrane of Rhodotorula glutinis. This carrier exhibited a km of 3.7 +/- 0.3 mM. D-Ribose was found to be a competitive inhibitor with a Ki of 19 +/- 1 mM. The results suggest that L-glucose is transported by the high-Km, D-ribose carrier. L-Glucose was transported against a concentration gradient and the transport was inhibited by the proton conductor 2,4-dinitrophenol.

摘要

在720倍的糖浓度范围内研究了粘红酵母转运L-葡萄糖的动力学。对饱和等温线的分析表明，粘红酵母质膜中存在一种L-葡萄糖单载体系统。该载体的米氏常数为3.7±0.3 mM。发现D-核糖是一种竞争性抑制剂，其抑制常数为19±1 mM。结果表明，L-葡萄糖是由高米氏常数的D-核糖载体转运的。L-葡萄糖逆浓度梯度转运，且该转运受到质子导体2,4-二硝基苯酚的抑制。