Bogyo D, Vaidya N, Flashner M, Tanenbaum S W
Department of Chemistry, SUNY College of Environmental Science & Forestry, Syracuse 13210.
Biochimie. 1988 Feb;70(2):187-91. doi: 10.1016/0300-9084(88)90060-0.
Treatment of purified Ehrlich ascites cell plasma membranes either with [3H]cytochalasin B or [3H]19-O-acetylchaetoglobosin A under photolytic conditions produced several radioactive polypeptides which were characterized by SDS-PAGE analyses. The major proteins so photolabeled were in the 60,000-80,000 Da range, with less labeling found in polypeptides smaller than 43,000 and greater than 90,000 Da. Immunofluorescent staining failed to identify the major photolabeled component as actin. It is concluded, in keeping with prior investigations using other cell types, that the predominant proteins photolabeled by cytochalasins are affiliated with the glucose-transport system.
在光解条件下，用[3H]细胞松弛素B或[3H]19 - O - 乙酰毛壳球蛋白A处理纯化的艾氏腹水癌细胞质膜，产生了几种放射性多肽，通过SDS - PAGE分析对其进行了表征。如此光标记的主要蛋白质分子量在60,000 - 80,000 Da范围内，在小于43,000 Da和大于90,000 Da的多肽中标记较少。免疫荧光染色未能将主要的光标记成分鉴定为肌动蛋白。与之前使用其他细胞类型的研究一致，得出的结论是，细胞松弛素光标记的主要蛋白质与葡萄糖转运系统有关。
