艾氏腹水瘤细胞表面抗RNA抗体结合位点的分布

Distribution of anti-RNA antibody-binding sites on the surfaces of Ehrlich ascites tumor cells.

作者信息

Terasaki T

出版信息

Cell Struct Funct. 1984 Jun;9(2):181-5. doi: 10.1247/csf.9.181.

DOI:10.1247/csf.9.181

PMID:6209022

Abstract

The distribution of anti-RNA antibody-binding sites on the surfaces of Ehrlich ascites tumor cells was studied by membrane immunofluorescence after binding anti-RNA antibody on the cell surfaces. Results showed that the cells formed caps after incubation with anti-RNA antibody at 4 degrees C and the elevation of their temperature to 37 degrees C. Pronase treatment of the cell surfaces completely inhibited reactivity with anti-RNA antibody. These results suggest that the RNAs on the surfaces of Ehrlich ascites tumor cells are linked to membrane protein membrane-bound cytoskeleton complexes.

摘要

通过在艾氏腹水癌细胞表面结合抗RNA抗体后进行膜免疫荧光研究，对艾氏腹水癌细胞表面抗RNA抗体结合位点的分布进行了研究。结果显示，细胞在4℃与抗RNA抗体孵育并升温至37℃后形成帽状物。用链霉蛋白酶处理细胞表面可完全抑制其与抗RNA抗体的反应性。这些结果表明，艾氏腹水癌细胞表面的RNA与膜蛋白膜结合细胞骨架复合物相连。