Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, Doha 2713, Qatar; Biomedical Research Center, Qatar University, Doha 2713, Qatar.
Pushpagiri Research Centre, Pushpagiri Institute of Medical Sciences, Tiruvalla, Kerala 689 101, India.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109801. doi: 10.1016/j.msec.2019.109801. Epub 2019 May 27.
In situ tissue engineering is emerging as a novel approach in tissue engineering to repair damaged tissues by boosting the natural ability of the body to heal itself. This can be achieved by providing suitable signals and scaffolds that can augment cell migration, cell adhesion on the scaffolds and proliferation of endogenous cells that facilitate the repair. Lack of appropriate cell proliferation and angiogenesis are among the major issues associated with the limited success of in situ tissue engineering during in vivo studies. Exploitation of metal oxide nanoparticles such as yttrium oxide (YO) nanoparticles may open new horizons in in situ tissue engineering by providing cues that facilitate cell proliferation and angiogenesis in the scaffolds. In this context, YO nanoparticles were synthesized and incorporated in polycaprolactone (PCL) scaffolds to enhance the cell proliferation and angiogenic properties. An optimum amount of YO-containing scaffolds (1% w/w) promoted the proliferation of fibroblasts (L-929) and osteoblast-like cells (UMR-106). Results of chorioallantoic membrane (CAM) assay and the subcutaneous implantation studies in rats demonstrated the angiogenic potential of the scaffolds loaded with YO nanoparticles. Gene expression study demonstrated that the presence of YO in the scaffolds can upregulate the expression of cell proliferation and angiogenesis related biomolecules such as VEGF and EGFR. Obtained results demonstrated that YO nanoparticles can perform a vital role in tissue engineering scaffolds to promote cell proliferation and angiogenesis.
原位组织工程作为组织工程学中的一种新方法，通过增强机体自身的愈合能力来修复受损组织。这可以通过提供合适的信号和支架来实现，这些信号和支架可以促进细胞迁移、细胞在支架上的黏附和内源性细胞的增殖，从而促进修复。在体内研究中，原位组织工程的成功受到限制，其中一个主要问题是缺乏适当的细胞增殖和血管生成。利用氧化钇（YO）等金属氧化物纳米粒子可以为原位组织工程开辟新的前景，为支架中的细胞增殖和血管生成提供线索。在这种情况下，合成了 YO 纳米粒子并将其掺入聚己内酯（PCL）支架中，以增强细胞增殖和血管生成特性。含有适量 YO 的支架（1%w/w）促进了成纤维细胞（L-929）和类成骨细胞（UMR-106）的增殖。绒毛尿囊膜（CAM）分析和大鼠皮下植入研究的结果表明，负载 YO 纳米粒子的支架具有血管生成潜力。基因表达研究表明，支架中存在 YO 可以上调与细胞增殖和血管生成相关的生物分子如 VEGF 和 EGFR 的表达。所得结果表明，YO 纳米粒子可以在组织工程支架中发挥重要作用，促进细胞增殖和血管生成。
