Patel Dinesh K, Seo Yu-Ri, Dutta Sayan Deb, Lim Ki-Taek
The Institute of Forest Science, Kangwon National University Chuncheon 24341 Republic of Korea.
Department of Biosystems Engineering, College of Agriculture and Life Sciences, Kangwon National University Chuncheon 24341 Republic of Korea
RSC Adv. 2019 Nov 5;9(62):36040-36049. doi: 10.1039/c9ra06260b. eCollection 2019 Nov 4.
Cellulose nanocrystals (CNCs) have received a great amount of attention to the production of micro/nano-platforms for tissue engineering applications. CNCs were extracted from rice husk biomass and characterized by different spectroscopic techniques. The biocompatibility of the extracted CNCs was revealed by the WST-1 assay technique in the presence of human mesenchymal stem cells (hMSCs) after different time intervals. An improvement in the mechanical properties was observed in the fabricated scaffolds (PCL/CNC) compared to PCL scaffolds. Graphene oxide (GO)-coated (PCL/CNC) electrospun scaffolds (GPC) were prepared by the deposition of PCL/CNC composite nanofibers on the surface of GO for tissue engineering. Notably, better cell proliferation and differentiation were observed in the presence of the fabricated scaffolds. This enhancement of the properties of the fabricated scaffolds was due to the presence of conductive GO moieties which facilitated the cellular response. Therefore, the fabricated materials have the potential to be used as a biomaterial for enhanced cell proliferation and osteogenic differentiation.
纤维素纳米晶体（CNCs）在用于组织工程应用的微/纳米平台生产方面受到了广泛关注。从稻壳生物质中提取了CNCs，并通过不同的光谱技术对其进行了表征。在不同时间间隔后，通过WST-1检测技术在人间充质干细胞（hMSCs）存在的情况下揭示了提取的CNCs的生物相容性。与聚己内酯（PCL）支架相比，在制备的支架（PCL/CNC）中观察到机械性能有所改善。通过将PCL/CNC复合纳米纤维沉积在氧化石墨烯（GO）表面来制备用于组织工程的GO涂层（PCL/CNC）电纺支架（GPC）。值得注意的是，在制备的支架存在的情况下观察到了更好的细胞增殖和分化。制备的支架性能的这种增强归因于导电GO部分的存在，其促进了细胞反应。因此，制备的材料有潜力用作增强细胞增殖和成骨分化的生物材料。
