Department of Biotechnology, Indian Institute of Technology Roorkee, India.
Department of Biotechnology, Indian Institute of Technology Roorkee, India; Center for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109834. doi: 10.1016/j.msec.2019.109834. Epub 2019 May 30.
Bioactive polymers are highly used polymers for preparing electrospun nanofiber based scaffold in the field of wound dressing to treat chronic non-healing wounds. Here, we report, the fabrication and evaluation of Carica papaya incorporated poly (vinyl) alcohol (PVA) blended gelatin nanofibers. PVA/Gelatin/Carica papaya nanofibrous scaffold was fabricated by electrospinning method. The obtained nanofibrous scaffold was characterized by using various analytical techniques such as FTIR, XRD, TEM, FESEM, AFM, and TGA. The average diameter of these nanofibers was found in the range 140-160 nm using FESEM. This scaffold showed excellent antibacterial activity against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria. The hemocompatibility was analyzed using platelet adhesion test. The cytotoxic activity of these nanofibrous scaffold against fibroblast cells (NIH 3T3) was studied and found no cytotoxic effect. Therefore, these results substantiated that Carica papaya loaded PVA/Gelatin nanofibrous scaffold could be a promising candidate for wound healing application.
生物活性聚合物是高度使用的聚合物，用于制备基于电纺纳米纤维的支架在伤口敷料领域治疗慢性难愈伤口。在这里，我们报告了掺入聚（乙烯醇）（PVA）的木瓜（Carica papaya）共混明胶纳米纤维的制造和评价。通过静电纺丝法制备了 PVA/明胶/木瓜纳米纤维支架。使用各种分析技术，如 FTIR、XRD、TEM、FESEM、AFM 和 TGA 对所获得的纳米纤维支架进行了表征。使用 FESEM 发现这些纳米纤维的平均直径在 140-160nm 范围内。该支架对革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性大肠杆菌（E. coli）两种细菌均表现出优异的抗菌活性。使用血小板黏附试验分析了血液相容性。研究了这些纳米纤维支架对成纤维细胞（NIH 3T3）的细胞毒性作用，发现没有细胞毒性作用。因此，这些结果证实，负载木瓜的 PVA/明胶纳米纤维支架可能是伤口愈合应用的有前途的候选物。
