粪肠球菌蛋白水解性转接合子中溶血素性状的表达

Expression of the hemolysin trait in a proteolytic transconjugant of Streptococcus faecalis.

作者信息

Casas I A, Marquez E, Zimmerman L N

机构信息

Depart. de Biologia, Facultad de Ciencias Veterinarias, Universidad del Zulia, Maracaibo, Venezuela.

出版信息

Biochimie. 1988 Feb;70(2):283-5. doi: 10.1016/0300-9084(88)90072-7.

DOI:10.1016/0300-9084(88)90072-7

PMID:3134952

Abstract

Streptococcus faecalis 31H-1 is a proteolytic transconjugant of strain 31B, that harbors the 38.5 Mdal plasmid which codes for hemolysin in strain X14. It fails to express the hemolytic character in broth due to its proteolytic character. Hemolytic activity can be demonstrated when the proteolytic activity is inhibited by 10(-4) M EDTA. The effect of erythromycin (on the donor cells) and of proteinase K during conjugation suggests that the conjugation system involving the 38.5 Mdal plasmid of S. faecalis strain X14 is sex-pherormone mediated.

摘要

粪肠球菌31H - 1是31B菌株的蛋白水解转接合体，它含有38.5兆道尔顿的质粒，该质粒在X14菌株中编码溶血素。由于其蛋白水解特性，它在肉汤中不能表达溶血特性。当蛋白水解活性被10^(-4) M的乙二胺四乙酸（EDTA）抑制时，可证明溶血活性。红霉素（对供体细胞）和蛋白酶K在接合过程中的作用表明，涉及粪肠球菌X14菌株38.5兆道尔顿质粒的接合系统是由性信息素介导的。