Houvet D, Clerc M
Med Trop (Mars). 1979 Jan-Feb;39(1):107-9.
The authors describe a paper blotting technique to reveal starch electrophoresis. After 3 minutes of contact between the paper band and the starch gel the former can be either dried during 5' at 90 degrees C then stained with nigrosine or sliced and revealed by an immunserum. This quick technique has been successfully applied to alpha-1-antitrypsin; on the one side phenotypes can be identified 24 hours earlier than with staining method using amido black 10 B and on the other side quality control of the fractionning can be done before the second stage of the Laurell type two-dimension separation.
作者描述了一种用于揭示淀粉电泳的纸印迹技术。在纸带与淀粉凝胶接触3分钟后，前者可在90℃下干燥5分钟，然后用尼罗蓝染色，或者切片并用免疫血清进行显色。这种快速技术已成功应用于α-1-抗胰蛋白酶；一方面，与使用氨基黑10B的染色方法相比，可提前24小时鉴定表型，另一方面，可在劳雷尔型二维分离的第二阶段之前进行分级分离的质量控制。
