Banco Español de Algas, FPCT de la Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, 35215, Telde, Gran Canaria, Spain.
IU-ECOAQUA, Grupo en Biodiversidad y Conservación, Universidad de Las Palmas de Gran Canaria, 35017, Las Palmas, Canary Islands, Spain.
Harmful Algae. 2019 Jul;87:101634. doi: 10.1016/j.hal.2019.101634. Epub 2019 Jun 28.
The suitability of the 'artificial substrate' method, i.e. standardized surfaces of fiberglass screens, for the quantification of four benthic harmful algal bloom (BHAB) dinoflagellates (Gambierdiscus, Ostreopsis, Prorocentrum and Coolia) was tested relative to estimates from natural macroalgal substrates. Sampling took place in a variety of intertidal and subtidal coastal habitats under different water motion conditions, at depths from 1 to 7 m, in two archipelagos of the Macaronesia region: The Canary Islands and Cape Verde. An immersion time of 24 h was sufficient to adequately estimate dinoflagellate abundances. Seven replicates were established as the ideal replication level, considering both reproducibility and sampling effort. In most cases, cell abundances of the four dinoflagellate genera showed lower variability on artificial substrates than on macroalgae, leading to more reliable estimates of abundances. The ratio of mean cell abundances on artificial substrates to mean cell abundances on macroalgae highly varied among sampling sites for each genus. This was especially true for Ostreopsis and Coolia. Thus, given the potentially harmful nature of benthic dinoflagellates, the transformation of abundances expressed as cells g of macroalgae to abundances expressed as cells cm is risky, and it should not be attempted in monitoring and management programs of harmful microalgae. In summary, results of this study support the use of artificial substrates in monitoring programs of BHAB dinoflagellates, while the risks of using macroalgae are stressed.
我们测试了“人工基质”方法（即玻璃纤维筛的标准化表面）对四种底栖有害藻华（BHAB）甲藻（Gambierdiscus、Ostreopsis、Prorocentrum 和 Coolia）定量的适用性，将其与天然大型海藻基质的估计值进行了比较。在 Macaronesia 地区的两个群岛（加那利群岛和佛得角群岛），我们在不同的水动力条件下，于潮间带和亚潮带的各种沿海栖息地，在 1 至 7 米的深度进行了采样。24 小时的浸泡时间足以充分估计甲藻的丰度。考虑到可重复性和采样工作量，我们确定了 7 个重复作为理想的复制水平。在大多数情况下，四种甲藻属的细胞丰度在人工基质上的变化小于在大型海藻上的变化，从而得出更可靠的丰度估计值。每个属在人工基质上的平均细胞丰度与在大型海藻上的平均细胞丰度的比值在每个采样点之间变化很大。这对于 Ostreopsis 和 Coolia 尤其如此。因此，鉴于底栖甲藻可能具有危害性，将以大型海藻 g 表示的细胞丰度转换为以 cm 表示的细胞丰度的做法存在风险，在有害微藻的监测和管理计划中不应尝试这种做法。总之，这项研究的结果支持在 BHAB 甲藻的监测计划中使用人工基质，同时强调了使用大型海藻的风险。
