MARBEC, Université de Montpellier, CNRS, IFREMER, IRD, 87 Avenue Jean Monnet, 34200 Sète, France.
MARBEC, Université de Montpellier, CNRS, IFREMER, IRD, Place Eugène Bataillon, 34095 Montpellier, France.
Toxins (Basel). 2023 Mar 11;15(3):217. doi: 10.3390/toxins15030217.
is an emerging benthopelagic neuro-toxic dinoflagellate species responsible for seasonal Pinnatoxins and Portimines contaminations of shellfish and marine animals. This species is challenging to detect in the environment, as it is present in low abundance and difficult to be identified using light microscopy. In this work, we developed a method using artificial substrates coupled with qPCR (AS-qPCR) to detect in a marine environment. This sensitive, specific and easy-to-standardize alternative to current techniques does not require specialized expertise in taxonomy. After determining the limits and specificity of the qPCR, we searched for the presence of in four French Mediterranean lagoons using artificial substrates collected every two weeks for one year. The AS-qPCR method revealed its occurrences in summer 2021 in every studied lagoon and detected cells in more samples than light microscopy. As development induces shellfish contamination even at low microalga densities, the AS-qPCR method is accurate and relevant for monitoring in a marine environment.
是一种新兴的底栖浮游神经毒素甲藻，负责贝类和海洋动物的季节性 Pinnatoxins 和 Portimines 污染。该物种在环境中难以检测，因为它的丰度低，并且很难使用光学显微镜进行识别。在这项工作中，我们开发了一种使用人工基质结合 qPCR（AS-qPCR）的方法来检测海洋环境中的 。与当前技术相比，这种灵敏、特异且易于标准化的替代方法不需要在分类学方面具有专业知识。在确定 qPCR 的限制和特异性后，我们使用每两周收集一次、为期一年的人工基质在法国四个地中海泻湖中寻找 的存在。AS-qPCR 方法显示，在 2021 年夏季，每个研究泻湖都有 的存在，并且比光学显微镜检测到更多的样本中有细胞存在。由于 的发育即使在低微藻密度下也会导致贝类污染，因此 AS-qPCR 方法是监测海洋环境中 的准确且相关的方法。
