Guangxi Key Laboratory of the Rehabilitation and Reconstruction of Oral and Maxillofacial Research; Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment; Guangxi Clinical Research Center for Craniofacial Deformity, College of Stomatology, Guangxi Medical University, Nanning, P.R. China.
School of Information and Management, Guangxi Medical University, Nanning, P.R. China.
Cancer Epidemiol Biomarkers Prev. 2019 Oct;28(10):1668-1681. doi: 10.1158/1055-9965.EPI-18-1122. Epub 2019 Jul 26.
Blood contains exosomes that are related to tumor cells. Those exosomes can regulate communication between cells and have a great influence on a variety of tumor-associated proceedings through their target cells. Therefore, serum exosomes (SE) were supposed to play a crucial role in cancer development.
This study presented a quantitative proteomics analysis to identify the protein content in SEs including 30 subjects from oral squamous cell carcinoma (OSCC) patients with lymph node metastasis (LNM), OSCC patients with no LNM (NLNM), and healthy controls (HC). Differentially expressed proteins (DEP) were analyzed by bioinformatics, and then a total of 30 subjects were used for Western blot and 60 subjects for IHC, ELISA, and RT-PCR verifications. The correlations were assessed between DEP expression and clinicopathologic factors.
A total of 415 proteins were identified. Comparing with HC and OSCC-NLNM, we found 37 proteins and 28 proteins in the SEs of OSCC-LNM, respectively. There were significant correlations among the expression of PF4V1 with tumor differentiation level, PF4V1 and F13A1 with the number of positive nodes, and ApoA1 with smoking and drinking. ROC curve analysis indicated that the combinations of the different biomarkers or specimen were obviously superior to single biomarker or specimen for diagnosing OSCC-LNM.
We conclude that PF4V1, CXCL7, F13A1, and ApoA1 from SEs may be related to the metastasis of OSCC, which would be helpful in the diagnosis of OSCC-LNM.
Biomarkers from SEs could help with the diagnosis of metastasis in OSCC.
血液中含有与肿瘤细胞相关的外泌体。这些外泌体可以调节细胞间的通讯，并通过靶细胞对各种与肿瘤相关的过程产生重大影响。因此，血清外泌体（SE）被认为在癌症发展中起关键作用。
本研究采用定量蛋白质组学分析方法，鉴定了 30 例来自口腔鳞状细胞癌（OSCC）伴淋巴结转移（LNM）患者、OSCC 无 LNM（NLNM）患者和健康对照者（HC）SE 中的蛋白质含量。通过生物信息学分析差异表达蛋白（DEP），然后使用 Western blot 对 30 例患者、免疫组化（IHC）、酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）对 60 例患者进行验证。评估 DEP 表达与临床病理因素之间的相关性。
共鉴定出 415 种蛋白质。与 HC 和 OSCC-NLM 相比，OSCC-LNM 的 SE 中分别发现了 37 种和 28 种蛋白质。PF4V1 与肿瘤分化程度、PF4V1 和 F13A1 与阳性淋巴结数以及 ApoA1 与吸烟和饮酒之间存在显著相关性。ROC 曲线分析表明，不同生物标志物或标本的组合在诊断 OSCC-LNM 方面明显优于单一生物标志物或标本。
我们得出结论，SE 中的 PF4V1、CXCL7、F13A1 和 ApoA1 可能与 OSCC 的转移有关，这有助于 OSCC-LNM 的诊断。
SE 中的生物标志物有助于 OSCC 转移的诊断。
