氯的拯救作用。
Chloride to the rescue.
机构信息
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803
出版信息
J Biol Chem. 2019 Jul 26;294(30):11402-11403. doi: 10.1074/jbc.H119.009687.
Abstract
On the fiftieth anniversary of the discovery of the Ser-His-Asp catalytic triad, perhaps the most unusual variation on the textbook classic is described: An incomplete catalytic triad in a hydrolase is rescued by a chloride ion (Fig. 1). Structural and functional data provide compelling evidence that the active site of a phospholipase from employs the anion in place of the commonly observed Asp, reminding us that even well-trodden scientific ground has surprises in store.
摘要
在发现丝氨酸-组氨酸-天冬氨酸催化三联体五十周年之际，人们描述了教科书中经典范例最不寻常的变化：在水解酶中，一个不完全的催化三联体被氯离子挽救（图 1）。结构和功能数据提供了令人信服的证据，表明来自 的一种磷脂酶的活性位点使用阴离子代替常见的天冬氨酸，这提醒我们，即使是经过充分探索的科学领域也有惊喜在等待着我们。
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