Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Biol Reprod. 2019 Oct 25;101(4):733-747. doi: 10.1093/biolre/ioz136.
The seminal vesicles can be infected by microorganisms, thereby resulting in vesiculitis and impairment in male fertility. Innate immune responses in seminal vesicles cells to microbial infections, which facilitate vesiculitis, have yet to be investigated. The present study aims to elucidate pattern recognition receptor-mediated innate immune responses in seminal vesicles epithelial cells. Various pattern recognition receptors, including Toll-like receptor 3, Toll-like receptor 4, cytosolic ribonucleic acid, and deoxyribonucleic acid sensors, are abundantly expressed in seminal vesicles epithelial cells. These pattern recognition receptors can recognize their respective ligands, thus activating nuclear factor kappa B and interferon regulatory factor 3. The pattern recognition receptor signaling induces expression of pro-inflammatory cytokines, such as tumor necrosis factor alpha (Tnfa) and interleukin 6 (Il6), chemokines monocyte chemoattractant protein-1 (Mcp1) and C-X-C motif chemokine 10 (Cxcl10), and type 1 interferons Ifna and Ifnb. Moreover, pattern recognition receptor-mediated innate immune responses up-regulated the expression of microsomal prostaglandin E synthase and cyclooxygenase 2, but they down-regulated semenogelin-1 expression. These results provide novel insights into the mechanism underlying vesiculitis and its impact on the functions of the seminal vesicles.
精囊可被微生物感染，从而导致精囊炎和男性生育能力受损。精囊细胞对微生物感染的固有免疫反应，有利于精囊炎的发生，但尚未得到研究。本研究旨在阐明上皮细胞中模式识别受体介导的固有免疫反应。多种模式识别受体，包括 Toll 样受体 3、Toll 样受体 4、细胞质核糖核酸和脱氧核糖核酸传感器，在上皮细胞中大量表达。这些模式识别受体可以识别各自的配体，从而激活核因子 kappa B 和干扰素调节因子 3。模式识别受体信号诱导促炎细胞因子如肿瘤坏死因子 α（TNFa）和白细胞介素 6（IL6）、趋化因子单核细胞趋化蛋白-1（Mcp1）和 C-X-C 基序趋化因子 10（Cxcl10）以及 I 型干扰素 Ifna 和 Ifnb 的表达。此外，模式识别受体介导的固有免疫反应上调了微粒体前列腺素 E 合酶和环氧化酶 2 的表达，但下调了精液蛋白 1 的表达。这些结果为精囊炎的发病机制及其对精囊功能的影响提供了新的见解。
