AVT - Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.
DWI - Leibniz Institute for Interactive Materials, RWTH Aachen University Forckenbeckstraße 50, 52074 Aachen, Germany.
Bioresour Technol. 2019 Nov;291:121821. doi: 10.1016/j.biortech.2019.121821. Epub 2019 Jul 16.
The aim of this work was to study ammonium-limited fed-batch conditions in heterotrophic C. vulgaris shake flask cultivations. Therefore, an innovative polymer-based ammonium release technique (polymer beads) was developed. Using these beads in shake flasks, C. vulgaris cultivations resulted in simultaneous growth and lipid accumulation. Lipid productivity was increased by 43% compared to batch cultivations. Furthermore, by online monitoring of the metabolic activity (RAMOS technique), unlimited growth and depletion of nutrients could be identified. A previously unknown sulfur limitation was detected in the applied Bold's Basal Medium. Combining the ammonium release beads with the RAMOS technique proved to be an efficient method for microalgae process development.
本工作旨在研究异养小球藻摇瓶培养中的铵限制补料条件。因此，开发了一种创新的基于聚合物的铵释放技术（聚合物珠）。在摇瓶中使用这些珠粒，小球藻培养可实现同步生长和脂质积累。与分批培养相比，脂质生产力提高了 43%。此外，通过代谢活性的在线监测（RAMOS 技术），可以识别出无限制的生长和营养物质的耗尽。在应用的 Bold's 基础培养基中检测到了以前未知的硫限制。将铵释放珠与 RAMOS 技术相结合被证明是一种有效的微藻工艺开发方法。
