采用酶联免疫吸附测定法评估抗单核细胞增生李斯特菌抗体。
Evaluation of anti-Listeria monocytogenes antibodies by ELISA.
作者信息
Mastroeni P, Leonardi M S, Gazzara D, Zummo S, Bizzini B
机构信息
Istituto di Microbiologia, Università di Messina, Italy.
出版信息
G Batteriol Virol Immunol. 1986 Jul-Dec;79(7-12):183-6.
PMID:3135221
Abstract
A new ELISA test using the purified Listeria monocytogenes-derived antigen LM 84 Ag is described together with its application in the diagnosis of listeriosis. Fifty-seven human sera were titrated by the ELISA and the complement fixation method and compared for specificity and sensitivity. The problem of possible cross-reactions with other antigens is discussed.
摘要
本文描述了一种使用纯化的单核细胞增生李斯特菌衍生抗原LM 84 Ag的新型酶联免疫吸附测定（ELISA）及其在李斯特菌病诊断中的应用。采用ELISA和补体结合法对57份人血清进行滴定，并比较其特异性和敏感性。文中还讨论了与其他抗原可能发生交叉反应的问题。
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