Debets J M, Van der Linden C J, Dieteren I E, Leeuwenberg J F, Buurman W A
Department of General Surgery, University of Limburg, Maastricht, The Netherlands.
J Immunol. 1988 Aug 15;141(4):1197-201.
In this study it was demonstrated that cross-linking of FcR on human monocytes induces the secretion of the cytotoxic and immunoregulatory cytokine TNF. Both soluble and insoluble immune complexes, solid-phase antibody and antibody-coated phagocytizable particles were used to cross-link FcR on monocytes. It was observed that monocytes secreted large amounts of TNF in each of these instances. Kinetic studies performed with soluble immune complexes showed that TNF was secreted very rapidly, e.g., within 2 h after addition of immune complexes to monocytes. These findings are relevant for the understanding of FcR-mediated immune responses by monocytes and macrophages, for example antibody-dependent cellular cytotoxicity and phagocytosis, and for disease states associated with circulating or tissue-fixed immune complexes.
在本研究中，已证明人单核细胞上FcR的交联可诱导细胞毒性和免疫调节细胞因子TNF的分泌。可溶性和不溶性免疫复合物、固相抗体以及抗体包被的可吞噬颗粒均被用于使单核细胞上的FcR交联。观察到在上述每种情况下单核细胞均分泌大量TNF。用可溶性免疫复合物进行的动力学研究表明，TNF分泌非常迅速，例如，在向单核细胞中加入免疫复合物后2小时内。这些发现对于理解单核细胞和巨噬细胞的FcR介导的免疫反应（例如抗体依赖性细胞毒性和吞噬作用）以及与循环或组织固定免疫复合物相关的疾病状态具有重要意义。
